substrate-driven convergence of the microbial community in lignocellulose-amended enrichments of gut microflora from the canadian beaver (castor canadensis) and north american moose (alces americanus). | strategic enrichment of microcosms derived from wood foragers can facilitate the discovery of key microbes that produce enzymes for the bioconversion of plant fiber (i.e., lignocellulose) into valuable chemicals and energy. in this study, lignocellulose-degrading microorganisms from the digestive systems of canadian beaver (castor canadensis) and north american moose (alces americanus) were enriched under methanogenic conditions for over 3 years using various wood-derived substrates, including ( ... | 2016 | 27446004 |
evaluation of two quantitative pcr assays using bacteroidales and mitochondrial dna markers for tracking dog fecal contamination in waterbodies. | this study describes a comparative performance evaluation of two qpcr assays targeting a dog-associated bacteroidales 16s rrna genetic marker (canbac-ucd) and a dog mitochondrial dna (mtdna) marker. the same fecal and environmental samples were assayed for the two markers thereby allowing direct comparison. a wide range of non-target species including, human, pig, horse, deer, mountain goat, bison, caribou, and moose were tested. marker persistence was also monitored in freshwater microcosms. bo ... | 2012 | 23041493 |
photolytic clean-up of biological samples for gas chromatographic analysis of chlorinated paraffins. | a method based on gas chromatography electron capture detection (gc-ecd) for the analysis of chlorinated paraffins (cps) in biological samples has been investigated. the method includes photolytic destruction of halogenated aromatic compounds, such as pcbs, to eliminate some of the interferences in the analysis of cps in environmental samples. gel permeation chromatography was used to isolate cps from the interfering components of toxaphene and chlordane after the photolysis. gc-ecd gave a detec ... | 2004 | 14664836 |
mineral licks as environmental reservoirs of chronic wasting disease prions. | chronic wasting disease (cwd) is a fatal neurodegenerative disease of deer, elk, moose, and reindeer (cervids) caused by misfolded prion proteins. the disease has been reported across north america and recently discovered in northern europe. transmission of cwd in wild cervid populations can occur through environmental routes, but limited ability to detect prions in environmental samples has prevented the identification of potential transmission "hot spots". we establish widespread cwd prion con ... | 2018 | 29719000 |