| genetic and serologic analysis of black creek canal virus and its association with human disease and sigmodon hispidus infection. | black creek canal (bcc) virus is a newly identified virus associated with hantavirus pulmonary syndrome (hps) in the southeastern united states. nucleotide sequences were determined for the complete s and m and partial l genomic segments of a bcc virus isolate. phylogenetic analysis indicates that each of the virus segments is unique, and there is no evidence of genetic reassortment having occurred between this and other previously characterized hantaviruses. all hantavirus-seropositive, wild-ca ... | 1995 | 7618284 |
| genetic and serologic analysis of black creek canal virus and its association with human disease and sigmodon hispidus infection. | black creek canal (bcc) virus is a newly identified virus associated with hantavirus pulmonary syndrome (hps) in the southeastern united states. nucleotide sequences were determined for the complete s and m and partial l genomic segments of a bcc virus isolate. phylogenetic analysis indicates that each of the virus segments is unique, and there is no evidence of genetic reassortment having occurred between this and other previously characterized hantaviruses. all hantavirus-seropositive, wild-ca ... | 1995 | 7618284 |
| isolation of black creek canal virus, a new hantavirus from sigmodon hispidus in florida. | numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (hps) case in dade county, florida. cotton rats (sigmodon hispidus) were the most frequently capture rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. hantavirus genome rna was detected in all the seropositive cotton rats tested, using a reverse transcriptase-polymerase chain reaction (rt-pcr) assay. a virus was isolated from tissues ... | 1995 | 7623004 |
| isolation of black creek canal virus, a new hantavirus from sigmodon hispidus in florida. | numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (hps) case in dade county, florida. cotton rats (sigmodon hispidus) were the most frequently capture rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. hantavirus genome rna was detected in all the seropositive cotton rats tested, using a reverse transcriptase-polymerase chain reaction (rt-pcr) assay. a virus was isolated from tissues ... | 1995 | 7623004 |
| hantavirus pulmonary syndrome in florida: association with the newly identified black creek canal virus. | hantavirus pulmonary syndrome (hps) is a recently recognized viral zoonosis. the first recognized cases were caused by a newly described hantavirus. sin nombre virus (previously known as muerto canyon virus), isolated from peromyscus maniculatus (deer mouse). we describe a 33-year-old floridian man who resided outside the ecologic range of p maniculatus but was found to have serologic evidence of a hantavirus infection during evaluation of azotemia associated with adult respiratory distress synd ... | 1996 | 8579086 |
| hantavirus pulmonary syndrome in florida: association with the newly identified black creek canal virus. | hantavirus pulmonary syndrome (hps) is a recently recognized viral zoonosis. the first recognized cases were caused by a newly described hantavirus. sin nombre virus (previously known as muerto canyon virus), isolated from peromyscus maniculatus (deer mouse). we describe a 33-year-old floridian man who resided outside the ecologic range of p maniculatus but was found to have serologic evidence of a hantavirus infection during evaluation of azotemia associated with adult respiratory distress synd ... | 1996 | 8579086 |
| polarized entry and release in epithelial cells of black creek canal virus, a new world hantavirus. | black creek canal (bcc) virus is a newly identified hantavirus from florida which is carried by the cotton rat (sigmodon hispidus) and is associated with hantavirus pulmonary syndrome (hps). we have investigated the interaction of bcc virus with polarized epithelial cells to examine whether entry and release of this virus occur at specific plasma membrane domains. the polarized vero c1008 monkey kidney cell line was grown on permeable filters and infected with bcc virus either through the apical ... | 1997 | 8995636 |
| polarized entry and release in epithelial cells of black creek canal virus, a new world hantavirus. | black creek canal (bcc) virus is a newly identified hantavirus from florida which is carried by the cotton rat (sigmodon hispidus) and is associated with hantavirus pulmonary syndrome (hps). we have investigated the interaction of bcc virus with polarized epithelial cells to examine whether entry and release of this virus occur at specific plasma membrane domains. the polarized vero c1008 monkey kidney cell line was grown on permeable filters and infected with bcc virus either through the apical ... | 1997 | 8995636 |
| genetic reassortment among viruses causing hantavirus pulmonary syndrome. | in order to determine the frequency and characteristics of reassortment among viruses causing hantavirus pulmonary syndrome (hps), mixed infections were initiated in tissue culture by using two closely related strains of sin nombre virus, cc107 (from eastern california) and nmr11 (from new mexico), which share the same species of rodent host in nature, the deer mouse (peromyscus maniculatus). potential reassortant virus plaques were screened by multiplex rt-pcr, using primers specific for indivi ... | 1998 | 9501041 |
| genetic reassortment among viruses causing hantavirus pulmonary syndrome. | in order to determine the frequency and characteristics of reassortment among viruses causing hantavirus pulmonary syndrome (hps), mixed infections were initiated in tissue culture by using two closely related strains of sin nombre virus, cc107 (from eastern california) and nmr11 (from new mexico), which share the same species of rodent host in nature, the deer mouse (peromyscus maniculatus). potential reassortant virus plaques were screened by multiplex rt-pcr, using primers specific for indivi ... | 1998 | 9501041 |
| role of actin microfilaments in black creek canal virus morphogenesis. | we have investigated the involvement of cytoskeletal proteins in the morphogenesis of black creek canal virus (bccv), a new world hantavirus. immunofluorescent staining of bccv-infected cells revealed a filamentous pattern of virus antigen, the appearance of which was sensitive to treatment with cytochalasin d, an actin microfilament-depolymerizing drug. double immunofluorescence staining of bccv-infected vero cells with anti-bccv nucleocapsid (n) monoclonal antibody and phalloidin revealed a co ... | 1998 | 9525606 |
| pathogenesis of a north american hantavirus, black creek canal virus, in experimentally infected sigmodon hispidus. | this report describes the first detailed analysis of the replication, persistence, and excretion of a north american hantavirus in its natural rodent reservoir. black creek canal virus was isolated from sigmodon hispidus (cotton rat) shortly after the identification of a hantavirus pulmonary syndrome (hps) case occurring in southern florida. six-week-old male cotton rats were inoculated subcutaneously with 1,000 tissue culture infectious doses. viral complementary rna (vcrna) was quantified as a ... | 1998 | 9684629 |
| pathogenesis of a north american hantavirus, black creek canal virus, in experimentally infected sigmodon hispidus. | this report describes the first detailed analysis of the replication, persistence, and excretion of a north american hantavirus in its natural rodent reservoir. black creek canal virus was isolated from sigmodon hispidus (cotton rat) shortly after the identification of a hantavirus pulmonary syndrome (hps) case occurring in southern florida. six-week-old male cotton rats were inoculated subcutaneously with 1,000 tissue culture infectious doses. viral complementary rna (vcrna) was quantified as a ... | 1998 | 9684629 |
| black creek canal virus infection in sigmodon hispidus in southern florida. | a total of 1,500 small mammals were collected and tested for antibodies cross-reactive to sin nombre virus (hantavirus: bunyaviridae) at 89 sites in a 1,600 km2 study area of southern florida. more than 95% of the 123 seropositive animals were cotton rats (sigmodon hispidus), suggesting infection by black creek canal virus, although seroreactive rattus rattus (5 of 294) and peromyscus gossypinus (1 of 39) also were captured. crude seroprevalence in s. hispidus was 11%. seroprevalence increased w ... | 1998 | 9840584 |
| black creek canal virus infection in sigmodon hispidus in southern florida. | a total of 1,500 small mammals were collected and tested for antibodies cross-reactive to sin nombre virus (hantavirus: bunyaviridae) at 89 sites in a 1,600 km2 study area of southern florida. more than 95% of the 123 seropositive animals were cotton rats (sigmodon hispidus), suggesting infection by black creek canal virus, although seroreactive rattus rattus (5 of 294) and peromyscus gossypinus (1 of 39) also were captured. crude seroprevalence in s. hispidus was 11%. seroprevalence increased w ... | 1998 | 9840584 |
| transmission of black creek canal virus between cotton rats. | black creek canal (bcc) virus is a hantavirus associated with hantavirus pulmonary syndrome in southeastern north america. the virus was isolated from the spleen of a cotton rat (sigmodon hispidus) trapped in southern florida. our previous studies have shown that we could consistently infect male cotton rats with bcc virus in the laboratory. these animals became persistently infected and virus could be detected in salivary glands, urine, and feces. in this report we show: (1) female and male cot ... | 2000 | 10568766 |
| transmission of black creek canal virus between cotton rats. | black creek canal (bcc) virus is a hantavirus associated with hantavirus pulmonary syndrome in southeastern north america. the virus was isolated from the spleen of a cotton rat (sigmodon hispidus) trapped in southern florida. our previous studies have shown that we could consistently infect male cotton rats with bcc virus in the laboratory. these animals became persistently infected and virus could be detected in salivary glands, urine, and feces. in this report we show: (1) female and male cot ... | 2000 | 10568766 |
| hantavirus nucleocapsid protein is expressed as a membrane-associated protein in the perinuclear region. | black creek canal virus (bccv) is a new world hantavirus which is associated with hantavirus pulmonary syndrome. we have examined the site of expression of the bccv nucleocapsid protein (nbccv) in the absence of bccv glycoproteins and found that the majority of the protein is localized to the golgi region. immunofluorescence analysis of bhk21 cells expressing the nbccv and la crosse virus nucleocapsid protein (nlacv) showed different intracellular localization patterns of these proteins within t ... | 2001 | 11160679 |
| new and old world hantaviruses differentially utilize host cytoskeletal components during their life cycles. | recently we reported that the n protein of the old world hantavirus, hantaan (htnv), traffics on microtubules to the er-golgi intermediate compartment (ergic) prior to its movement to the golgi and requires an intact ergic for viral replication. we have extended these studies to the new world hantaviruses, andes virus (andv) and black creek canal virus (bccv), and an additional old world hantavirus, seoul virus (seov). these studies support microtubule-dependent trafficking of the n protein to e ... | 2008 | 18234268 |
| pathology of black creek canal virus infection in juvenile hispid cotton rats (sigmodon hispidus). | the purpose of this study was to assess the effect of inoculum dose on the pathogenesis of black creek canal virus (bccv) infection in the hispid cotton rat (sigmodon hispidus), the principal host of bccv. no sign of illness was observed in any of the 52 juvenile hispid cotton rats inoculated with 3.1, 1.1, -0.9, or -2.9 log(10) median infectious doses(veroe6) (id(50-veroe6)) of bccv and euthanized on day 9, 18, 27, or 54 postinoculation (pi). analysis of virus assay and serological data indicat ... | 2010 | 20455779 |
| pathology of black creek canal virus infection in juvenile hispid cotton rats (sigmodon hispidus). | the purpose of this study was to assess the effect of inoculum dose on the pathogenesis of black creek canal virus (bccv) infection in the hispid cotton rat (sigmodon hispidus), the principal host of bccv. no sign of illness was observed in any of the 52 juvenile hispid cotton rats inoculated with 3.1, 1.1, -0.9, or -2.9 log(10) median infectious doses(veroe6) (id(50-veroe6)) of bccv and euthanized on day 9, 18, 27, or 54 postinoculation (pi). analysis of virus assay and serological data indicat ... | 2010 | 20455779 |
| development of a serotyping enzyme-linked immunosorbent assay system based on recombinant truncated hantavirus nucleocapsid proteins for new world hantavirus infection. | new world hantaviruses were divided into five groups based on the amino acid sequence variability of the internal variable region (around 230-302 amino acids) of hantavirus nucleocapsid protein (np). sin nombre virus (snv), andes virus, black creek canal virus (bccv), carrizal virus (carv) and cano delgadito virus belong to groups 1, 2, 3, 4 and 5, respectively. patient and rodent sera were serotyped successfully by an enzyme-linked immunosorbent assay (elisa) with recombinant truncated np lacki ... | 2012 | 22722226 |
| twenty-year summary of surveillance for human hantavirus infections, united states. | in the past 20 years of surveillance for hantavirus in humans in the united states, 624 cases of hantavirus pulmonary syndrome (hps) have been reported, 96% of which occurred in states west of the mississippi river. most hantavirus infections are caused by sin nombre virus, but cases of hps caused by bayou, black creek canal, monongahela, and new york viruses have been reported, and cases of domestically acquired hemorrhagic fever and renal syndrome caused by seoul virus have also occurred. rare ... | 2013 | 24274585 |
| muleshoe virus and other hantaviruses associated with neotomine or sigmodontine rodents in texas. | the broad objective of this study was to increase our knowledge of muleshoe virus and other hantaviruses associated with cricetid rodents in texas. anti-hantavirus antibody was found in 38 (3.2%) of 1171 neotomine rodents and 6 (1.8%) of 332 sigmodontine rodents from 10 texas counties; hantaviral rna was detected in 23 (71.9%) of 32 antibody-positive rodents. analyses of nucleocapsid protein gene sequences indicated muleshoe virus infection in four hispid cotton rats (sigmodon hispidus) from nor ... | 2017 | 28714801 |
| muleshoe virus and other hantaviruses associated with neotomine or sigmodontine rodents in texas. | the broad objective of this study was to increase our knowledge of muleshoe virus and other hantaviruses associated with cricetid rodents in texas. anti-hantavirus antibody was found in 38 (3.2%) of 1171 neotomine rodents and 6 (1.8%) of 332 sigmodontine rodents from 10 texas counties; hantaviral rna was detected in 23 (71.9%) of 32 antibody-positive rodents. analyses of nucleocapsid protein gene sequences indicated muleshoe virus infection in four hispid cotton rats (sigmodon hispidus) from nor ... | 2017 | 28714801 |
| a lethal disease model for new world hantaviruses using immunosuppressed syrian hamsters. | hantavirus, the hemorrhagic causative agent of two clinical diseases, is found worldwide with variation in severity, incidence and mortality. the most lethal hantaviruses are found on the american continent where the most prevalent viruses like andes virus and sin nombre virus are known to cause hantavirus pulmonary syndrome. new world hantavirus infection of immunocompetent hamsters results in an asymptomatic infection except for andes virus and maporal virus; the only hantaviruses causing a le ... | 2017 | 29077702 |
| a lethal disease model for new world hantaviruses using immunosuppressed syrian hamsters. | hantavirus, the hemorrhagic causative agent of two clinical diseases, is found worldwide with variation in severity, incidence and mortality. the most lethal hantaviruses are found on the american continent where the most prevalent viruses like andes virus and sin nombre virus are known to cause hantavirus pulmonary syndrome. new world hantavirus infection of immunocompetent hamsters results in an asymptomatic infection except for andes virus and maporal virus; the only hantaviruses causing a le ... | 2017 | 29077702 |