| development of a taqman-based real-time rt-pcr assay for the detection of sads-cov associated with severe diarrhea disease in pigs. | swine acute diarrhea syndrome coronavirus (sads-cov) is a novel coronavirus which was first reported in southern china in 2017. it can cause severe diarrhea disease in pigs. in order to detect this new emerging virus rapidly and reliably, a taqman-based real-time rt-pcr assay was established in this study. specific primers and probe were designed and synthesized based on the conserved region within the n gene of the viral genome. results showed that the lowest limit of detection was 3.0 × 101 co ... | 2018 | 29427670 |
| molecular diversity of coronavirus host cell entry receptors. | coronaviruses are a group of viruses causing disease in a wide range of animals including humans. since 2002, the successive emergence of bat-borne severe acute respiratory syndrome coronavirus (sars-cov), middle east respiratory syndrome coronavirus (mers-cov), swine acute diarrhea syndrome coronavirus (sads-cov) and sars-cov-2 have reinforced efforts in uncovering the molecular and evolutionary mechanisms governing coronavirus cell tropism and interspecies transmission. decades of studies have ... | 2020 | 33118022 |
| cryo-electron microscopy structure of the swine acute diarrhea syndrome coronavirus spike glycoprotein provides insights into evolution of unique coronavirus spike proteins. | coronaviruses (cov) have caused a number of major epidemics in humans and animals, including the current pandemic of coronavirus disease 2019 (covid-19), which has brought a renewed focus on the evolution and interspecies transmission of coronaviruses. swine acute diarrhea syndrome coronavirus (sads-cov), which was recently identified in piglets in southern china, is an alphacoronavirus that originates from the same genus of horseshoe bats as severe acute respiratory syndrome cov (sars-cov) and ... | 2020 | 32817223 |
| broad cross-species infection of cultured cells by bat hku2-related swine acute diarrhea syndrome coronavirus and identification of its replication in murine dendritic cells in vivo highlight its potential for diverse interspecies transmission. | outbreaks of severe diarrhea in neonatal piglets in guangdong, china, in 2017 resulted in the isolation and discovery of a novel swine enteric alphacoronavirus (seacov) derived from the species rhinolophus bat coronavirus hku2 (y. pan, x. tian, p. qin, b. wang, et al., vet microbiol 211:15-21, 2017). seacov was later referred to as swine acute diarrhea syndrome cov (sads-cov) by another group (p. zhou, h. fan, t. lan, x.-l. yang, et al., nature 556:255-258, 2018). the present study was set up to ... | 2019 | 31554686 |
| increasing the translation of mouse models of mers coronavirus pathogenesis through kinetic hematological analysis. | newly emerging viral pathogens pose a constant and unpredictable threat to human and animal health. coronaviruses (covs) have a penchant for sudden emergence, as evidenced by severe acute respiratory syndrome coronavirus (sars-cov), middle east respiratory syndrome cov (mers-cov) and most recently, swine acute diarrhea syndrome coronavirus (sads-cov). small animal models of emerging viral pathogenesis are crucial to better understand the virus and host factors driving disease progression. howeve ... | 2019 | 31339932 |
| swine acute diarrhea syndrome coronavirus-induced apoptosis is caspase- and cyclophilin d- dependent. | swine acute diarrhea syndrome coronavirus (sads-cov), a newly discovered enteric coronavirus, is the aetiological agent that causes severe clinical diarrhea and intestinal pathological damage in piglets. to understand the effect of sads-cov on host cells, we characterized the apoptotic pathways and elucidated mechanisms underlying the process of apoptotic cell death after sads-cov infection. sads-cov-infected cells showed evidence of apoptosis in vitro and in vivo. the use of a pan-caspase inhib ... | 2020 | 32090691 |
| swine acute diarrhea syndrome coronavirus (sads-cov) antagonizes interferon-β production via blocking ips-1 and rig-i. | swine acute diarrhea syndrome coronavirus (sads-cov), a newly emerging enteric coronavirus, is considered to be associated with swine acute diarrhea syndrome (sads) which has caused significantly economic losses to the porcine industry. interactions between sads-cov and the host innate immune response is unclear yet. in this study, we used ipec-j2 cells as a model to explore potential evasion strategies employed by sads-cov. our results showed that sads-cov infection failed to induce ifn-β produ ... | 2020 | 31884203 |
| significant inhibition of re-emerged and emerging swine enteric coronavirus in vitro using the multiple shrna expression vector. | swine enteric coronaviruses (secovs), including porcine epidemic diarrhea virus (pedv), swine acute diarrhea syndrome coronavirus (sads-cov), and porcine deltacoronavirus (pdcov) have emerged and been prevalent in pig populations in china for the last several years. however, current traditional inactivated and attenuated pedv vaccines are of limited efficacy against circulating pedv variants, and there are no commercial vaccines for prevention of pdcov and sads-cov. rna interference (rnai) is a ... | 2019 | 30905822 |
| epitope mapping and cellular localization of swine acute diarrhea syndrome coronavirus nucleocapsid protein using a novel monoclonal antibody. | a swine acute diarrhea syndrome coronavirus (sads-cov) that causes severe diarrhea in suckling piglets was identified in southern china in 2017. to develop an antigen that is specific, sensitive, and easy to prepare for serological diagnosis, antigenic sites in the sads-cov nucleocapsid (n) protein were screened. we generated and characterized an n-reactive monoclonal antibody (mab) 3e9 from mice immunized with recombinant n protein. through fine epitope mapping of mab 3e9 using a panel of eukar ... | 2019 | 31518629 |
| characterization of a novel bat-hku2-like swine enteric alphacoronavirus (seacov) infection in cultured cells and development of a seacov infectious clone. | swine enteric alphacoronavirus (seacov), also known as swine acute diarrhea syndrome coronavirus (sads-cov), belongs to the species rhinolophus bat coronavirus hku2. herein, we report on the primary characterization of seacov in vitro. four antibodies against the seacov spike, membrane, nucleocapsid and nonstructural protein 3 capable of reacting with viral antigens in seacov-infected vero cells were generated. we established a dna-launched seacov infectious clone based on the cell adapted passa ... | 2019 | 31419711 |
| microfluidic-rt-lamp chip for the point-of-care detection of emerging and re-emerging enteric coronaviruses in swine. | porcine epidemic diarrhea virus (pedv), porcine deltacoronavirus (pdcov), and swine acute diarrhea syndrome-coronavirus (sads-cov) are three emerging and re-emerging coronaviruses (covs). symptoms caused by these three viruses are extremely similar, including acute diarrhea, vomiting and even death in piglets. to date, strict biosecurity is still the most effective disease prevention and control measures, and the early detection of pathogens is the most important link. here, we developed a micro ... | 2020 | 32674781 |
| development of a taqman-probe-based multiplex real-time pcr for the simultaneous detection of emerging and reemerging swine coronaviruses. | with the outbreak of the recent severe acute respiratory syndrome coronavirus 2 (sars-cov-2) in 2019, coronaviruses have become a global research hotspot in the field of virology. coronaviruses mainly cause respiratory and digestive tract diseases, several coronaviruses are responsible for porcine diarrhea, such as porcine epidemic diarrhea virus (pedv), porcine deltacoronavirus (pdcov), and emerging swine acute diarrhea syndrome coronavirus (sads-cov). those viruses have caused huge economic lo ... | 2020 | 32490723 |
| prevalence and phylogenetic analysis of porcine diarrhea associated viruses in southern china from 2012 to 2018. | in china, large-scale outbreaks of severe diarrhea caused by viruses have occurred in pigs since late 2010. to investigate the prevalence and genetic evolution of diarrhea-associated viruses responsible for the outbreaks, a total of 2987 field diarrheal samples collected from 168 pig farms in five provinces in southern china during 2012-2018 were tested. | 2019 | 31881873 |
| emerging and re-emerging coronaviruses in pigs. | porcine epidemic diarrhea virus (pedv), porcine deltacoronavirus (pdcov), and swine acute diarrhea syndrome-coronavirus (sads-cov) are emerging/reemerging coronaviruses (covs). they cause acute gastroenteritis in neonatal piglets. sequence analyses suggest that pedv and sads-cov may have originated from bat covs and pdcov from a sparrow cov, reaffirming the interspecies transmission of covs. the clinical signs and pathogenesis of the three viruses are similar. necrosis of infected intestinal epi ... | 2019 | 30654269 |
| development of a real time reverse transcription loop-mediated isothermal amplification method (rt-lamp) for detection of a novel swine acute diarrhea syndrome coronavirus (sads-cov). | a novel swine acute diarrhea syndrome coronavirus (sads-cov) that causes severe diarrhea in suckling piglets was identified in southern china in 2017. a simple and rapid detection test was developed for this virus using real-time rt-lamp based on the conserved n gene of the virus. the method had a detection limit of 1.0 × 101 copies/μl with no cross-reactions with classical swine fever virus, porcine and respiratory syndrome virus na, porcine and respiratory syndrome virus eu, transmissible gast ... | 2018 | 29936095 |
| swine enteric alphacoronavirus (swine acute diarrhea syndrome coronavirus): an update three years after its discovery. | discovered in 2017, swine enteric alphacoronavirus (seacov), also known as swine acute diarrhea syndrome coronavirus (sads-cov) or porcine enteric alphacoronavirus (peav), is the fifth porcine cov identified in diarrheal piglets. the presumed name "sads-cov" may not be appropriate since current studies have not provided strong evidence for high pathogenicity of the virus. seacov was the most recently recognized cov of potential bat origin prior to the novel human severe acute respiratory syndrom ... | 2020 | 32482591 |
| swine acute diarrhea syndrome coronavirus replication in primary human cells reveals potential susceptibility to infection. | zoonotic coronaviruses represent an ongoing threat, yet the myriads of circulating animal viruses complicate the identification of higher-risk isolates that threaten human health. swine acute diarrhea syndrome coronavirus (sads-cov) is a newly discovered, highly pathogenic virus that likely evolved from closely related hku2 bat coronaviruses, circulating in rhinolophus spp. bats in china and elsewhere. as coronaviruses cause severe economic losses in the pork industry and swine are key intermedi ... | 2020 | 33046644 |
| rna-seq-based whole transcriptome analysis of ipec-j2 cells during swine acute diarrhea syndrome coronavirus infection. | the new emergence of swine acute diarrhea syndrome coronavirus (sads-cov) has resulted in high mortality in suckling pigs in china. to date, the transcriptional expression of host cells during sads-cov infection has not been documented. in this study, by means of rna-seq technology, we investigated the whole genomic expression profiles of intestinal porcine epithelial cells (ipec-j2) infected with a sads-cov strain sads-cov-ch-fjwt-2018. a total of 24,676 genes were identified: 23,677 were known ... | 2020 | 32903570 |
| structural and biochemical characterization of sads-cov papain-like protease 2. | swine acute diarrhea syndrome coronavirus (sads-cov) is a novel coronavirus that is involved in severe diarrhea disease in piglets, causing considerable agricultural and economic loss in china. the emergence of this new coronavirus increases the importance of understanding sads-cov as well as antivirals. coronaviral proteases, including main proteases and papain-like proteases (plp), are attractive antiviral targets because of their essential roles in polyprotein processing and thus viral matura ... | 2020 | 32216114 |
| attenuation of a virulent swine acute diarrhea syndrome coronavirus strain via cell culture passage. | swine acute diarrhea syndrome coronavirus (sads-cov) is a newly identified enteric alphacoronavirus that causes fatal diarrhea in newborn piglets in china. here, we propagated a virulent strain sads-cov/cn/gdwt/2017 in vero cells for up to 83 passages. four strains of sads-cov/gdwt-p7, -p18, -p48 and -p83 were isolated and characterized. sequence alignments showed that these four novel strains exhibited 16 nucleotide mutations and resultant 10 amino acid substitutions in open reading frame 1a/1b ... | 2019 | 31580972 |
| development of a sybr green-based real-time rt-pcr assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus. | swine acute diarrhea syndrome coronavirus (sads-cov) is a novel coronavirus which was associated with severe diarrhea disease in pigs. sads-cov was first detected and identified as the causative agent of a devastating swine disease outbreak in southern china in 2017. routine monitoring and early detection of the source of infection is therefore integral to the prevention and control of sads-cov infection. in this study, a sybr green-based real-time reverse transcription quantitative polymerase c ... | 2019 | 30593837 |
| complete genome sequence of a novel swine acute diarrhea syndrome coronavirus, ch/fjwt/2018, isolated in fujian, china, in 2018. | the full-length genome sequence of a novel swine acute diarrhea syndrome coronavirus (sads-cov), ch/fjwt/2018, was determined, which was genetically most closely related to cn/gdwt/2017, recently discovered in fujian, china. the indel sites of the spike (s) gene of ch/fjwt/2018 were most similar to those of bat-origin sads-related coronaviruses. | 2018 | 30533848 |