| antigenic variation of european haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodies. | monoclonal antibodies (mabs) against puumala (puu) virus, the aetiological agent of nephropathia epidemica, were produced by fusing activated spleen cells from a bank vole (clethrionomys glareolus) with the mouse myeloma cell line sp2/0. this novel approach, utilizing the natural vector of puu virus for hybridoma production, proved to be highly efficient, and eight stable puu virus-specific heterohybridomas were isolated and characterized. the bank vole mabs were all specific for the nucleocapsi ... | 1991 | 1716651 |
| antigenic variation of european haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodies. | monoclonal antibodies (mabs) against puumala (puu) virus, the aetiological agent of nephropathia epidemica, were produced by fusing activated spleen cells from a bank vole (clethrionomys glareolus) with the mouse myeloma cell line sp2/0. this novel approach, utilizing the natural vector of puu virus for hybridoma production, proved to be highly efficient, and eight stable puu virus-specific heterohybridomas were isolated and characterized. the bank vole mabs were all specific for the nucleocapsi ... | 1991 | 1716651 |
| molecular characterization of the prospect hill virus m rna segment: a comparison with the m rna segments of other hantaviruses. | complementary dna representing the genomic m rna segment of the prospect hill (ph) hantavirus was cloned and its nucleotide sequence determined. the ph virus m rna segment is 3707 nucleotides in length and has a long open reading frame in the viral complementary-sense rna with a coding capacity of 1142 amino acids. the predicted gene product of the ph virus m segment was compared with the corresponding gene products of hantaan virus strain 76-118 (hantaan), sapporo rat virus strain sr-11 (sr) an ... | 1991 | 1840609 |
| nucleotide sequence analysis of the s genomic segment of prospect hill virus: comparison with the prototype hantavirus. | the genomic s rna segment of prospect hill virus (phv), a member of the hantavirus genus, was molecularly cloned and the nucleotide sequence of the cdna determined. the phv s rna segment is 1675 nucleotides long. a long open reading frame was identified in the viral complementary-sense rna that could encode a 433 amino acid (49k) nucleocapsid (n) protein. comparison with the sequence of the related hantavirus (hantaan 76-118) s rna segment indicated that there was 57% nucleotide sequence homolog ... | 1990 | 2309440 |
| serologic evidence of hantaviral infections within small mammal communities of baltimore, maryland: spatial and temporal patterns and host range. | serologic evidence was used to investigate the spatial and temporal distribution and host range of hantaviruses in small mammal communities in baltimore, md. immunofluorescent antibody (ifa) reactive to a baltimore rat isolate of seoul virus was detected in 44% of 404 norway rats captured at 4 park or residential locations; 21% of 121 meadow voles captured at 4 park locations possessed significant ifa titers to prospect hill virus. evidence from plaque neutralization assay of rodent sera suggest ... | 1989 | 2570533 |
| serologic evidence of hantaviral infections within small mammal communities of baltimore, maryland: spatial and temporal patterns and host range. | serologic evidence was used to investigate the spatial and temporal distribution and host range of hantaviruses in small mammal communities in baltimore, md. immunofluorescent antibody (ifa) reactive to a baltimore rat isolate of seoul virus was detected in 44% of 404 norway rats captured at 4 park or residential locations; 21% of 121 meadow voles captured at 4 park locations possessed significant ifa titers to prospect hill virus. evidence from plaque neutralization assay of rodent sera suggest ... | 1989 | 2570533 |
| serotypic classification of hantaviruses by indirect immunofluorescent antibody and plaque reduction neutralization tests. | antisera prepared against 16 strains of hantaviruses isolated from patients with hemorrhagic fever with renal syndrome (hfrs) or from rodents captured in hfrs-endemic and nonendemic regions were titrated against hantaan virus strain 76-118, puumala virus strain sotkamo, and prospect hill virus strain prospect hill-i by using the indirect immunofluorescent antibody and plaque reduction neutralization tests. isolates fell into one of four distinct groups or serotypes. serotype 1 included apodemus- ... | 1985 | 2866197 |
| partial characterization of a hantavirus isolated from a clethrionomys glareolus captured in belgium. | a hantavirus was isolated in vero-e6 cells from lungs of a free living bank vole (clethrionomys glareolus) captured in turnhout, province of antwerp--northern part of belgium. with help of monoclonal antibodies the belgian hantavirus isolate could be clearly differentiated from hantaan virus strain 76-118, prospect hill virus strain ph1 and sr11, a hantavirus isolated from laboratory wistar rat in japan, but not from the nephropathia epidemica virus strain hällnäs. | 1987 | 2886029 |
| partial characterization of a hantavirus isolated from a clethrionomys glareolus captured in belgium. | a hantavirus was isolated in vero-e6 cells from lungs of a free living bank vole (clethrionomys glareolus) captured in turnhout, province of antwerp--northern part of belgium. with help of monoclonal antibodies the belgian hantavirus isolate could be clearly differentiated from hantaan virus strain 76-118, prospect hill virus strain ph1 and sr11, a hantavirus isolated from laboratory wistar rat in japan, but not from the nephropathia epidemica virus strain hällnäs. | 1987 | 2886029 |
| antibody to hemorrhagic fever with renal syndrome viruses (hantaviruses) in the united states. | hantaviruses similar to those which cause hemorrhagic fever with renal syndrome have been isolated from rodents in the united states. serologic evidence suggests that these viruses infect humans. clinical disease has not, however, been associated with infection in the united states. to expand knowledge of the distribution of hantaviruses in the united states and attempt to identify a clinical syndrome associated with infection, a serologic survey was undertaken of us forestry service personnel a ... | 1987 | 2891295 |
| serological survey of prospect hill virus infection in indigenous wild rodents in the usa. | we found serological evidence of infection with prospect hill virus, a hantaan-like virus isolated from meadow voles (microtus pennsylvanicus), in microtine and cricetid rodents trapped in maryland, west virginia, minnesota and california, usa. fluorescent antibodies were detected in sera from m. pennsylvanicus (74/277), m. californicus (39/185), clethrionomys gapperi (5/51), peromyscus maniculatus (4/22) and p. truei (1/11). sera from seropositive p. maniculatus contained neutralizing antibodie ... | 1987 | 2895510 |
| serological survey of prospect hill virus infection in indigenous wild rodents in the usa. | we found serological evidence of infection with prospect hill virus, a hantaan-like virus isolated from meadow voles (microtus pennsylvanicus), in microtine and cricetid rodents trapped in maryland, west virginia, minnesota and california, usa. fluorescent antibodies were detected in sera from m. pennsylvanicus (74/277), m. californicus (39/185), clethrionomys gapperi (5/51), peromyscus maniculatus (4/22) and p. truei (1/11). sera from seropositive p. maniculatus contained neutralizing antibodie ... | 1987 | 2895510 |
| [use of monoclonal antibodies for differentiating strains of the virus causing the hemorrhagic fever with renal syndrome]. | using monoclonal antibody and animal immune sera the experiment confirmed the existence of antigenic relationships between the strains of virus of hemorrhagic fever with renal syndrome (hfrs) isolated in the ussr from clethrionomys glareolus and members of all known serotypes of hfrs virus. clear-cut differentiation was made from serotypes apodemus and rattus, and cross-relationships were shown between the strains isolated in the ussr and prospect hill virus (phv), a member of the serotype micro ... | 1987 | 3124351 |
| [use of monoclonal antibodies for differentiating strains of the virus causing the hemorrhagic fever with renal syndrome]. | using monoclonal antibody and animal immune sera the experiment confirmed the existence of antigenic relationships between the strains of virus of hemorrhagic fever with renal syndrome (hfrs) isolated in the ussr from clethrionomys glareolus and members of all known serotypes of hfrs virus. clear-cut differentiation was made from serotypes apodemus and rattus, and cross-relationships were shown between the strains isolated in the ussr and prospect hill virus (phv), a member of the serotype micro ... | 1987 | 3124351 |
| experimental hantavirus infection in nonhuman primates. | mild, transient proteinuria and azotemia were produced in three cynomolgus monkeys (macaca fascicularis) and a chimpanzee (pan troglodytes) following intravenous inoculation with prospect hill virus, a hantavirus isolated from meadow voles in the united states. this is the first demonstration of an acute nephropathy in nonhuman primates with the viruses causing hemorrhagic fever with renal syndrome. | 1988 | 3137914 |
| experimental hantavirus infection in nonhuman primates. | mild, transient proteinuria and azotemia were produced in three cynomolgus monkeys (macaca fascicularis) and a chimpanzee (pan troglodytes) following intravenous inoculation with prospect hill virus, a hantavirus isolated from meadow voles in the united states. this is the first demonstration of an acute nephropathy in nonhuman primates with the viruses causing hemorrhagic fever with renal syndrome. | 1988 | 3137914 |
| partial characterization of prospect hill virus isolated from meadow voles in the united states. | | 1985 | 3930622 |
| partial characterization of prospect hill virus isolated from meadow voles in the united states. | | 1985 | 3930622 |
| antigenic relatedness of pigeon herpes encephalomyelitis virus to other avian herpesviruses. | pigeon herpes encephalomyelitis virus (phev) was compared with seven avian herpesviruses for antigenic relatedness using monospecific antisera and the indirect fluorescent-antibody (ifa), agar-gel-immunodiffusion, and serum-neutralization tests. no antigenic relationship was detected between phev and marek's disease virus, turkey herpesvirus, infectious laryngotracheitis virus, and duck enteritis virus. a common precipitating antigen was detected between the phev and pigeon herpesvirus (phv), ow ... | 1983 | 6196014 |
| susceptibility of day-old chicks and ducklings, goslings and quails to pigeon herpes encephalomyelitis and pigeon herpesviruses. | day-old chicks were susceptible to pigeon herpes encephalomyelitis virus (phev) by intracerebral (i/c) inoculation. infected birds developed neurologic signs starting from 2 to 15 days post-infection, and 85% died. the virus was recovered from the brains of diseased chicks in titers ranging between 104 and 105.5 eid 50/0.2 ml. inoculated birds shed the virus in their droppings throughout the 2 weeks observation period. day-old chicks given the virus by the intranasal (i/n) or oral routes did not ... | 1983 | 6306904 |
| in vivo and in vitro effect of acyclovir on pseudorabies virus, infectious bovine rhinotracheitis virus and pigeon herpesvirus. | the effect of various concentrations of acyclovir on the mean plaque size of pseudorabies virus (shv), infectious bovine rhinotracheitis virus (ibr virus) and pigeon herpesvirus (phv) has been studied. acyclovir significantly reduced the mean plaque size of shv and phv, whereas ibr virus was less affected and did only show a reduction of the mean plaque size at the highest concentration of acyclovir used (1000 microm). in vivo effect of acyclovir was tested using pigeons and budgerigars experime ... | 1983 | 6318645 |
| monoclonal antibodies specific for hantaan virus. | six hybridoma cell line producing monoclonal antibodies to hantaan virus were established by fusion of ns-1 mouse myeloma cells with spleen cells of mice immunized with hantaan virus strain 76-118. the specificity of these monoclonal antibodies was established by immunoblotting analysis and immunofluorescence. five of the clones reacted with antigens on the cell surface and in the cytoplasm, and one clone reacted with a determinant expressed only in the cytoplasm of the infected cells. two of th ... | 1983 | 6408643 |
| prospect hill virus: serologic evidence for infection in mammologists. | | 1984 | 6425688 |
| characterization of human antibody responses to four corners hantavirus infections among patients with hantavirus pulmonary syndrome. | hantavirus pulmonary syndrome (hps) is a human disease caused by a newly identified hantavirus, which we will refer to as four corners virus (fcv). fcv is related most closely to puumala virus (puu) and to prospect hill virus (phv). twenty-five acute hps serum samples were tested for immunoglobulin g (igg) and igm antibody reactivities to fcv-encoded recombinant proteins in western blot (immunoblot) assays. all hps serum samples contained both igg and igm antibodies to the fcv nucleocapsid (n) p ... | 1994 | 7512156 |
| hantavirus pulmonary syndrome: the four corners disease. | to review the epidemiology, pathogenesis, clinical features, diagnosis, and treatment of hantavirus infections, focusing on the recent outbreak of hantavirus pulmonary syndrome in the us. | 1995 | 7711347 |
| genome structure and variability of a virus causing hantavirus pulmonary syndrome. | a previously unrecognized hantavirus (family bunyaviridae) has recently been detected and shown to be associated with a severe respiratory illness with high mortality, termed hantavirus pulmonary syndrome (hps). this disease has now been identified throughout the western united states. we present nucleotide sequence characterization of the three rna segments composing the hps virus genome and address the question of the apparent emergence of this highly lethal virus. no evidence of genetic reass ... | 1994 | 8178455 |
| a novel hantavirus associated with an outbreak of fatal respiratory disease in the southwestern united states: evolutionary relationships to known hantaviruses. | four corners hantavirus (fcv) is the tentative name of the suspected etiologic agent of the newly identified hantavirus-associated respiratory distress syndrome (hards). the identification in hards patients of serum immunoglobulin m and immunoglobulin g antibodies that cross-reacted with hantaan, seoul, and puumala virus antigens first suggested that fcv is a hantavirus. limited nucleotide sequence data from the fcv glycoprotein-2 (g2) confirmed that fcv is a hantavirus and showed that it is mos ... | 1994 | 8289363 |
| complete nucleotide sequence of pepper huasteco virus: analysis and comparison with bipartite geminiviruses. | the complete nucleotide sequence of pepper huasteco geminivirus (phv) isolated in northern mexico was determined. the phv genome consists of two circular ssdna molecules of 2631 bases (phv a) and 2589 bases (phv b). phv has a genome organization typical of a bipartite geminivirus with four open reading frames (orfs) (ar1, al1, al2 and al3) in component a and two (br1 and bl1) in component b. an unexpected orf was found in the complementary sense strand of phv a. this orf, termed al5, is found en ... | 1993 | 8409944 |
| isla vista virus: a genetically novel hantavirus of the california vole microtus californicus. | prospect hill virus (ph) was isolated from a meadow vole (microtus pennsylvanicus) in 1982, and much of its genome has been sequenced. hantaviruses of other new world microtine rodents have not been genetically characterized. we show that another microtus species (the california vole m. californicus) from the united states is host to a genetically distinct ph-like hantavirus, isla vista virus (ilv). the nucleocapsid protein of ilv differs from that of ph by 11.1% and a portion of the g2 glycopro ... | 1995 | 8847529 |
| isla vista virus: a genetically novel hantavirus of the california vole microtus californicus. | prospect hill virus (ph) was isolated from a meadow vole (microtus pennsylvanicus) in 1982, and much of its genome has been sequenced. hantaviruses of other new world microtine rodents have not been genetically characterized. we show that another microtus species (the california vole m. californicus) from the united states is host to a genetically distinct ph-like hantavirus, isla vista virus (ilv). the nucleocapsid protein of ilv differs from that of ph by 11.1% and a portion of the g2 glycopro ... | 1995 | 8847529 |
| the genomic 5' terminus of manchester calicivirus. | an enteric calicivirus showing the classic cup-shaped surface morphology was identified in a stool sample obtained from a child with symptoms of acute gastroenteritis (portishead virus, phv). genomic rna was extracted directly from the phv stool sample and amplified by rt-pcr using primers based on the manchester isolate of hucv. the 3' terminus of the cdna was defined by homopolymer tailing with datp and revealed an additional 165 nucleotides suggesting that the previously determined manchester ... | 1997 | 9354265 |
| beta3 integrins mediate the cellular entry of hantaviruses that cause respiratory failure. | newly emerged hantaviruses replicate primarily in the pulmonary endothelium, cause acute platelet loss, and result in hantavirus pulmonary syndrome (hps). we now report that specific integrins expressed on platelets and endothelial cells permit the cellular entry of hps-associated hantaviruses. infection with hps-associated hantaviruses, ny-1 and sin nombre virus (snv), is inhibited by antibodies to beta3 integrins and by the beta3-integrin ligand, vitronectin. in contrast, infection with the no ... | 1998 | 9618541 |
| antigenic and nucleotide characterization of a herpesvirus isolated from pacific harbor seals (phoca vitulina richardsii). | this report describes the antigenic and nucleotide characterization of a herpes-like virus that has been isolated from the adrenal tissues of neonatal pacific harbor seals. infection with this virus has been previously implicated as a major cause of death of animals undergoing rehabilitation. comparison and phylogenetic analysis of sequenced fragments of the dna polymerase, glycoprotein b and glycoprotein d genes, and immunofluorescence assay using herpesvirus-specific monoclonal antibodies, dem ... | 1998 | 9856089 |
| identification of a sequence element involved in ac2-mediated transactivation of the pepper huasteco virus coat protein gene. | the geminivirus ac2 gene product transactivates the expression of the coat and movement protein (cp and bv1) genes, and this effect seems to be mediated by specific although hitherto unknown cis-acting elements. in this work we examined regions from the cp and bv1 gene promoters of pepper huasteco virus (phv) to define the sequence elements involved in regulation by ac2. results from transient gene expression and transgenic plant assays suggest that a truncated 115-nt cp promoter is still respon ... | 1999 | 9918875 |
| functional expression of receptors for calcitonin gene-related peptide, calcitonin, and vasoactive intestinal peptide in the human thymus and thymomas from myasthenia gravis patients. | the molecular and functional expression of serpentine membrane receptors for vasoactive intestinal peptide (vip), calcitonin gene-related peptide (cgrp), and calcitonin (ct) were characterized in human thymus and thymomas from myasthenia gravis (mg) patients and thymic epithelial cells either in primary culture (ptec) or transformed by the simian virus 40 large t (sv40lt) oncogene (lt-tec). using rt-pcr combined with southern analysis, we identified the pcr products corresponding to the receptor ... | 1999 | 9973484 |
| otarine herpesvirus-1: a novel gammaherpesvirus associated with urogenital carcinoma in california sea lions (zalophus californianus). | the incidence of neoplasia in california sea lions (csls) is considered to be unusually high. electron microscopic examination of some of these urogenital tumours revealed the presence of virions with typical herpes-like structure. while current attempts to cultivate this virus have not been successful, molecular studies employing dna extracted from tumour tissues allowed both the classification of the agent and its identification in tumours and archived tissue samples. two genome fragments gene ... | 2002 | 11888696 |
| pathogenic and nonpathogenic hantaviruses differentially regulate endothelial cell responses. | hantaviruses cause two human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). hantaviruses infect human endothelial cells but cause little or no damage to the infected endothelium. we analyzed with affymetrix dna arrays (santa clara, ca) the endothelial cell transcriptional responses directed by hantaviruses associated with hps [new york-1 virus (ny-1v)], hfrs [hantaan virus (htnv)], or by a hantavirus not associated with human disease [prospect hil ... | 2002 | 12368479 |
| regulation of cellular gene expression in endothelial cells by sin nombre and prospect hill viruses. | mechanisms of hantavirus-induced vascular leakage remain unknown. this study was initiated to determine whether hantavirus-induced changes in endothelial cell gene expression may provide insight into disease mechanisms. additionally, by using pathogenic sin nombre virus (snv) and non-pathogenic prospect hill virus (phv), we wanted to identify cellular responses that are likely to differentiate pathogenic from nonpathogenic hantaviruses. using the affymetrix dna array, we found that phv and snv d ... | 2004 | 15279702 |
| antibodies to selected pathogens in free-ranging terrestrial carnivores and marine mammals in canada. | antibody titres to selected pathogens (canine adenovirus [cav-2], feline herpesvirus [fhv], phocine herpesvirus [phv-1], canine distemper virus, dolphin morbillivirus [dmv], phocine distemper virus [pdv], parainfluenza virus type 3 [pi3], rabies virus, dolphin rhabdovirus [drv], canine coronavirus, feline coronavirus, feline leukaemia virus, borrelia burgdorferi and toxoplasma gondii) were determined in whole blood or serum samples from selected free-ranging terrestrial carnivores and marine mam ... | 2004 | 15338705 |
| peptide antagonists that inhibit sin nombre virus and hantaan virus entry through the beta3-integrin receptor. | specific therapy is not available for the treatment of hantavirus cardiopulmonary syndrome caused by sin nombre virus (snv). the entry of pathogenic hantaviruses into susceptible human cells is dependent upon expression of the alpha(v)beta(3) integrin, and transfection of human beta(3) integrin is sufficient to confer infectibility onto cho (chinese hamster ovary) cells. furthermore, pretreatment of susceptible cells with anti-beta(3) antibodies such as c7e3 or its fab fragment reopro prevents h ... | 2005 | 15919886 |
| peptide antagonists that inhibit sin nombre virus and hantaan virus entry through the beta3-integrin receptor. | specific therapy is not available for the treatment of hantavirus cardiopulmonary syndrome caused by sin nombre virus (snv). the entry of pathogenic hantaviruses into susceptible human cells is dependent upon expression of the alpha(v)beta(3) integrin, and transfection of human beta(3) integrin is sufficient to confer infectibility onto cho (chinese hamster ovary) cells. furthermore, pretreatment of susceptible cells with anti-beta(3) antibodies such as c7e3 or its fab fragment reopro prevents h ... | 2005 | 15919886 |
| functional characterization of the geminiviral conserved late element (cle) in uninfected tobacco. | the conserved late element (cle) was originally identified as an evolutionarily conserved dna sequence present in geminiviral intergenic regions. cle has subsequently been observed in promoter sequences of bacterial (t-dna) and plant origin, suggesting a role in plant and plant viral gene regulation. synthetic dna cassettes harboring direct repeats of the cle motif were placed upstream from a -46 to +1 minimal camv 35s promoter-luciferase reporter gene and reporter activity characterized in nico ... | 2005 | 16021333 |
| molecular detection of a virus, pilchard herpesvirus, associated with epizootics in australasian pilchards sardinops sagax neopilchardus. | in 1995, and again in 1998, high mortalities of pilchards sardinops sagax neopilchardus were seen over their entire geographical range on the australian coastline. a virus with typical herpesvirus morphology was identified as the causative agent, although the source of the virus remains unknown. at the time of the mortality events, the only available diagnostic test available for the detection of the virus was electron microscopy and hence, development of a rapid diagnostic test for detection an ... | 2005 | 16465828 |
| the pathogenic ny-1 hantavirus g1 cytoplasmic tail inhibits rig-i- and tbk-1-directed interferon responses. | hantaviruses cause two diseases with prominent vascular permeability defects, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. all hantaviruses infect human endothelial cells, although it is unclear what differentiates pathogenic from nonpathogenic hantaviruses. we observed dramatic differences in interferon-specific transcriptional responses between pathogenic and nonpathogenic hantaviruses at 1 day postinfection, suggesting that hantavirus pathogenesis may in part be de ... | 2006 | 16973572 |
| andes and prospect hill hantaviruses differ in early induction of interferon although both can downregulate interferon signaling. | hantavirus pulmonary syndrome (hps) is a severe respiratory disease which is thought to result from a dysregulated immune response to infection with pathogenic hantaviruses, such as sin nombre virus or andes virus (andv). other new world hantaviruses, such as prospect hill virus (phv), have not been associated with human disease. activation of an antiviral state and cell signaling in response to hantavirus infection were examined using human primary lung endothelial cells, the main target cell i ... | 2007 | 17202220 |
| degrons at the c terminus of the pathogenic but not the nonpathogenic hantavirus g1 tail direct proteasomal degradation. | pathogenic hantaviruses cause two human diseases: hantavirus pulmonary syndrome (hps) and hemorrhagic fever with renal syndrome (hfrs). the hantavirus g1 protein contains a long, 142-amino-acid cytoplasmic tail, which in ny-1 virus (ny-1v) is ubiquitinated and proteasomally degraded (e. geimonen, i. fernandez, i. n. gavrilovskaya, and e. r. mackow, j. virol. 77: 10760-10768, 2003). here we report that the g1 cytoplasmic tails of pathogenic andes (hps) and hantaan (hfrs) viruses are also degraded ... | 2007 | 17267477 |
| the effect of subunit or modified live bovine herpesvirus-1 vaccines on the efficacy of a recombinant pasteurella haemolytica vaccine for the prevention of respiratory disease in feedlot calves. | the efficacy of a pasteurella haemolytica vaccine (phv) administered once to calves within 24 hours of arrival at a feedlot was tested for the ability to prevent morbidity and mortality from all bovine respiratory disease (brd) and specifically from fibrinous pneumonia mortality. the phv consisted of two immunizing ingredients: outer membrane proteins extracted from p. haemolytica, plus genetically attenuated leukotoxin produced by recombinant dna technology. this double blind study was conducte ... | 1992 | 17424116 |
| [molecular characterization of hantavirus zhejiang isolate zt10 strain from m. fartis]. | to learn about the complete genomic sequence of the seoul virus strain zt10 isolated from m. fartis. | 2008 | 18414685 |
| studies on reactivation and transmission of pigeon herpes virus (phv) for raising phv-free pigeons (columba livia dom.). | for raising pigeons free of herpes virus infection, 12 parental pigeons were monitored for shedding of pigeon herpes virus (phv) during 18 weeks of reproduction. in most of the birds only a sporadic virus excretion, which was never associated with clinical signs could be observed. however, three out of seven squabs raised by their parents became infected during the first 3 weeks of life and all seven became phv seropositive within 4 months. therefore, we raised seven squabs hatched in an incubat ... | 1997 | 18483950 |
| the ny-1 hantavirus gn cytoplasmic tail coprecipitates traf3 and inhibits cellular interferon responses by disrupting tbk1-traf3 complex formation. | pathogenic hantaviruses replicate within human endothelial cells and cause two diseases, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. in order to replicate in endothelial cells pathogenic hantaviruses inhibit the early induction of beta interferon (ifn-beta). expression of the cytoplasmic tail of the pathogenic ny-1 hantavirus gn protein is sufficient to inhibit rig-i- and tbk1-directed ifn responses. the formation of tbk1-traf3 complexes directs irf-3 phosphorylation ... | 2008 | 18614628 |
| [comparison of three strains of herpesvirus isolated from psittacine birds with the pigeon herpesvirus]. | two strains of herpesvirus isolated from psittacine birds in belgium (psihv/b/cu; vindevogel et al., 1978) (psihv/b/u; meulemans et al., 1978) and a third one isolated in switzerland (psihv/s/1355; ehrsam et al., 1978) were compared with the pigeon herpesvirus (strain phv/b/cu1; vindevogel et al., 1975). on chick embryo fibroblasts, the cytopathic effect of strain psihv/b/cu was focal whereas strains psihv/b/u and psihv/s/1355 showed a diffuse cytopathic effect. also, under agarose overlay, the ... | 1980 | 18770276 |
| betac1, the pathogenicity factor of tylccnv, interacts with as1 to alter leaf development and suppress selective jasmonic acid responses. | viruses induce pathogenic symptoms on plants but the molecular basis is poorly understood. here, we show that transgenic arabidopsis expressing the pathogenesis protein betac1 of tomato yellow leaf curl china virus (tylccnv), a geminivirus, can phenocopy to a large extent disease symptoms of virus-infected tobacco plants in having upward curled leaves, radialized leaves with outgrowth tissues from abaxial surfaces, and sterile flowers. these morphological changes are paralleled by a reduction in ... | 2008 | 18794352 |
| interactions between geminiviruses in a naturally occurring mixture: pepper huasteco virus and pepper golden mosaic virus. | abstract pepper huasteco virus (phv) and pepper golden mosaic virus (pepgmv) are found in mixtures in many horticultural crops in mexico. this combination constitutes an interesting, naturally occurring model system to study several aspects of virus-virus interactions. possible interactions between phv and pepgmv were studied at four levels: symptom expression, gene expression, replication, and movement. in terms of symptom expression, the interaction was shown to be host-dependent because antag ... | 2003 | 18944336 |
| complementation of coat protein mutants of pepper huasteco geminivirus in transgenic tobacco plants. | abstract the role of the pepper huasteco virus (phv) coat protein (cp) gene during the infection was investigated in three different hosts by using mutations that produced truncated proteins and by complementation assays in transgenic plants. the infectivity analysis revealed that mutants that express truncated cp (cp7 and cp191) behave like the wild-type virus when inoculated onto pepper and nicotiana benthamiana plants in terms of symptom expression and viral dna movement. on the contrary, the ... | 1999 | 18944688 |
| comparison of three molecular methods for the detection of pilchard herpesvirus in archived paraffin-embedded tissue and frozen tissue. | two epizootics affecting pilchards sardinops sagax neopilchardus have been observed over their entire geographical range off the australian coastline. the first occurred in 1995, involving high mortality (at least 10%) that devastated the pilchard population. the second occurred in 1998 and involved even higher mortality (70%). both epizootics moved rapidly against the prevailing leeuwin and east australian currents from a defined point of origin. a herpesvirus, pilchard herpesvirus (phv), was d ... | 2008 | 19062751 |
| hantaan virus triggers tlr3-dependent innate immune responses. | immediately after viral infection, innate responses including expression of ifn-alpha/beta and ifn-stimulated genes (isgs) are elicited ubiquitously by recruitment of specific pathogen recognition receptors. the velocity to induce ifn-alpha/beta and isgs in response to an infection is often decisive for virulence. interestingly, in primary endothelial cells isgs are induced later by hantaviruses pathogenic to humans than those considered to be nonpathogenic or of low virulence. here we demonstra ... | 2009 | 19234180 |
| degradation and aggresome formation of the gn tail of the apathogenic tula hantavirus. | the cytoplasmic tails of envelope glycoprotein gn of pathogenic hantaviruses but not of the apathogenic prospect hill virus (phv) were recently reported to be proteasomally degraded in simian cos7 cells. here, we show that the cytoplasmic tails of the glycoproteins of the apathogenic hantaviruses tula virus (tulv) and phv are also degraded through the ubiquitin-proteasome pathway, both in human hek-293 and in simian vero e6 cells. tulv gn tails formed aggresomes in cells with proteasomal inhibit ... | 2009 | 19675185 |
| infection of in vivo differentiated human mast cells with hantaviruses. | increased vascular permeability is a key feature of the pathological symptoms caused by hantaviruses. here, we analysed the interaction between hantaviruses and mast cells, which regulate vascular homeostasis. in highly purified human skin mast cells increasing amounts of hantaan (htnv) and, to a lower extent, prospect hill (phv) virions were produced. replication was confirmed by the production of viral plus-strand rna as determined by a virus strand-specific rt-pcr. phv but not htnv elicited e ... | 2010 | 20071485 |
| generation and characterization of genetic reassortants between puumala and prospect hill hantavirus in vitro. | hantaviruses belong to the family bunyaviridae characterized by tri-segmented rna genomes. depending on the hantavirus species, infection can lead to hantavirus cardiopulmonary or haemorrhagic fever with renal syndrome. in vitro studies suggest that pathogenic hantaviruses evade induction of innate antiviral responses, and this ability might determine the virulence in humans. since reverse genetic systems are not available, in vitro reassortment is currently the only way to culture defined hanta ... | 2010 | 20505009 |
| the c-terminal 42 residues of the tula virus gn protein regulate interferon induction. | hantaviruses primarily infect the endothelial cell lining of capillaries and cause two vascular permeability-based diseases. the ability of pathogenic hantaviruses to regulate the early induction of interferon determines whether hantaviruses replicate in endothelial cells. tula virus (tulv) and prospect hill virus (phv) are hantaviruses which infect human endothelial cells but fail to cause human disease. phv is unable to inhibit early interferon (ifn) responses and fails to replicate within hum ... | 2011 | 21367904 |
| the structure of the hantavirus zinc finger domain is conserved and represents the only natively folded region of the gn cytoplasmic tail. | hantaviruses, of the family bunyaviridae, are present throughout the world and cause a variety of infections ranging from the asymptomatic to mild and severe hemorrhagic fevers. hantaviruses are enveloped anti-sense rna viruses that contain three genomic segments that encode for a nucleocapsid protein, two membrane glycoproteins (gn and gc), and an rna polymerase. recently, the pathogenicity of hantaviruses has been mapped to the carboxyl end of the 150 residue gn cytoplasmic tail. the gn tail h ... | 2011 | 22203819 |
| development of a loop-mediated isothermal amplification method for rapid detection of pigeon circovirus. | there are no effective antiviral treatments for pigeon circovirus (picv); thus, rapid diagnosis is critical for effective control of the disease caused by this virus. the recent development of a novel lamp technique that amplifies nucleic acids rapidly with high specificity and sensitivity under isothermal conditions has overcome some of the deficiencies of nucleic-acid-based diagnostic tests. we established a lamp method for rapid detection of picv using two pairs of primers that were designed ... | 2014 | 24193953 |
| hantaviruses induce cell type- and viral species-specific host microrna expression signatures. | the mechanisms of hantavirus-induced modulation of host cellular immunity remain poorly understood. recently, micrornas (mirnas) have emerged as a class of essential regulators of host immune response genes. to ascertain if differential host mirna expression toward representative hantavirus species correlated with immune response genes, mirna expression profiles were analyzed in human endothelial cells, macrophages and epithelial cells infected with pathogenic and nonpathogenic rodent- and shrew ... | 2013 | 24074584 |
| premalignant epithelial changes in the uterine cervix of women aged less than thirty with special emphasis on the coexistent condylomatous lesions. | one hundred and sixty two women aged less than 30 years with either a dysplastic change or carcinoma in situ of the squamous epithelium of the uterine cervix were histologically assessed with special emphasis on the detection of the recently described condylomatous lesions (the flat, inverted and the papillomatous ones) caused by human papilloma virus (phv). a total of 134 condylomatous (co) lesions were -- discovered, the flat type being the most frequent one (75.4%) followed in decreasing orde ... | 2017 | 28211420 |
| occurrence, characteristics and control of pigeon paramyxovirus type 1 in pigeons. | newcastle disease (nd) is a highly contagious and devastating viral disease of poultry and other birds that has a worldwide distribution. nd in pigeons is called paramyxovirosis and is caused by antigenic "pigeon variant" of the virus (pigeon paramyxovirus type 1, ppmv-1). during ppmv-1 infections, central nervous system symptoms and sometimes high mortality are observed. in the case of infection with viscerotropic strains which exhibit specific affinity for the kidneys, the first observed sign ... | 2014 | 24988870 |
| ssrna viruses from biotrophic oomycetes form a new phylogenetic group between nodaviridae and tombusviridae. | plasmopara halstedii virus (phv) is one of the few characterized oomycete viruses. although it is fully sequenced and well-studied in its genetic diversity, the exact classification and phylogenetic relationships of phv remain uncertain. the only known virus with characteristics similar to phv is the sclerophthora macrospora virus a (smv-a). both viruses infect obligate biotrophic oomycetes. while rna-dependent rna polymerases (rdrp) of oomycetes viruses have high similarity to the corresponding ... | 2017 | 28155196 |
| occurrence and genetic diversity of the plasmopara halstedii virus in sunflower downy mildew populations of the world. | plasmopara halstedii virus (phv) is a ss(+)rna virus that exclusively occurs in the sunflower downy mildew pathogen plasmopara halstedii, a biotrophic oomycete of severe economic impact. the virus origin and its genomic variability are unknown. a pcr-based screening of 128 samples of p. halstedii from five continents and up to 40 y old was conducted. phv rna was found in over 90 % of the isolates with no correlation to geographic origin or pathotype of its host. sequence analyses of the two open ... | 2015 | 25749368 |
| novel hybrid parvovirus-like virus, nih-cqv/phv, contaminants in silica column-based nucleic acid extraction kits. | | 2014 | 24335290 |
| the perils of pathogen discovery: origin of a novel parvovirus-like hybrid genome traced to nucleic acid extraction spin columns. | next-generation sequencing was used for discovery and de novo assembly of a novel, highly divergent dna virus at the interface between the parvoviridae and circoviridae. the virus, provisionally named parvovirus-like hybrid virus (phv), is nearly identical by sequence to another dna virus, nih-cqv, previously detected in chinese patients with seronegative (non-a-e) hepatitis. although we initially detected phv in a wide range of clinical samples, with all strains sharing ∼99% nucleotide and amin ... | 2013 | 24027301 |
| plasmopara halstedii virus causes hypovirulence in plasmopara halstedii, the downy mildew pathogen of the sunflower. | plasmopara halstedii virus (phv) is an isometric virus recently found in the oomycete plasmopara halstedii. the fully sequenced virus genome consists of two ss(+)rna strands encoding for the virus polymerase and the coat protein, respectively. most of previously screened field isolates of p. halstedii were found to harbor phv, but effects of phv on the pathogenicity and aggressiveness of the oomycete have not been investigated yet. to assess the influence of phv on the infectivity of p. halstedi ... | 2013 | 23747662 |
| distinct innate immune responses in human macrophages and endothelial cells infected with shrew-borne hantaviruses. | although hantaviruses have been previously considered as rodent-borne pathogens, recent studies demonstrate genetically distinct hantaviruses in evolutionarily distant non-rodent reservoirs, including shrews, moles and bats. the immunological responses to these newfound hantaviruses in humans are unknown. we compared the innate immune responses to imjin virus (mjnv) and thottapalayam virus (tpmv), two shrew-borne hantaviruses, with that toward two rodent-borne hantaviruses, pathogenic hantann vi ... | 2012 | 22944108 |