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toxigenic vibrio cholerae identified in estuaries of tanzania using pcr techniques.the current study assessed the occurrence of the vibrio cholerae serogroups o1 and o139 in environmental samples along salinity gradients in three selected estuaries of tanzania both through culture independent methods and by cultured bacteria. occurrence of v. cholerae was determined by pcr targeting the v. cholerae outer membrane protein gene ompw. furthermore, the presence of toxigenic strains and serogroups o1 and o139 was determined using multiplex pcr with specific primers targeting the ch ...201525743072
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