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studies on theileriidae (sporozoa) in tanzania. vii. additional note on the transmission of theileria parva.rhipicephalus evertsi successfully transmitted fatal east coast fever (theileria parva infection) in one experiment. a second one gave negative results, as did an attempted transmission of t. parva by amblyomma variegatum.1977407682
studies on theileriidae (sporozoa) in tanzania. x. a large-scale field trial on immunization against cattle theileriosis.50 cattle immunized by an infection and treatment method with 3 strains of theileria parva (mugaga, kiambu 5 and serengeti transformed), together with 19 controls, were exposed to natural tick infestation in tanzania, in a site where a continuous influx of infected ticks from different regions of the country may be expected to occur. exposure lasted for 2 months, monitoring continued for 3 more weeks after exposure ended. all 19 controls contracted east coast fever and died. 40 of the 50 immuniz ...1977414388
studies on theileriidae (sporozoa) in tanzania. iv. a field trial on immunization against east coast fever (theileria parva infection of cattle).seven cattle, immunized by an infection and treatment method with 3 strains of theileria parva (muguga, kiambu 5 and serengeti transformed), and five controls were exposed to natural tick infestation in tanzania, at a site where 2 precontrols had previously contracted fatal east coast fever within 3 weeks. all controls became infected with fatal ecf within a month of exposure. one immunized animal survived, two died from a theileria mutans infection and one from a babesia bigemina infection. the ...1976982549
severe anaemia due to haematopoietic precursor cell destruction in field cases of east coast fever in tanzania.examinations were made on erythrocytes, thrombocytes, leukocytes, lymph nodes, thymus, haemal nodes and bone marrow in field cases of east coast fever (ecf) in tanzania. seventy-six clinically sick short-horn zebu and taurine-zebu crosses, positive for theileria parva piroplasms and schizonts and 55 apparently healthy cattle were studied. the syndrome observed was characterised by severe pancytopenia, with massive normocytic, normochromic anaemia, panleukopenia and thrombocytopenia, but no retic ...19948073608
evaluation of pcr to detect theileria parva in field-collected tick and bovine samples in tanzania.the ability of pcr to detect infections of theileria parva, the cause of east coast fever, in field-collected tick and bovine samples from tanzania was evaluated. pcr-detected infection prevalence was high (15/20, 75%) in unfed adult rhipicephalus appendiculatus ticks that fed as nymphs on an acutely-infected calf, but low (22/836, 2.6%) in unfed adult r. appendiculatus collected from field sites in tanzania. tick infection prevalence was comparable to that in previous studies that used salivary ...200312591193
the immunisation of cattle against east coast fever (theileria parva infection) using tetracyclines: a review of the literature and a reappraisal of the method. 196514344196
an outbreak of east coast fever on the comoros: a consequence of the import of immunised cattle from tanzania?in 2003 and 2004, a severe epidemic decimated the cattle population on grand comore, the largest island of the union of comoros. fatalities started soon after the import of cattle from tanzania. theileria parva and its vector, rhipicephalus appendiculatus, could be identified as the main culprits of the epidemic. characterisation by multilocus genotyping revealed that the t. parva parasites isolated on the comoros were identical to the components of the muguga cocktail vaccine used in tanzania t ...200716996692
a review on prevalence, control measure, and tolerance of tanzania shorthorn zebu cattle to east coast fever in tanzania.in tanzania, control of east coast fever (ecf) has predominantly relied on tick control using acaricides and chemotherapy, little on ecf vaccination, and very little on dissemination regarding animal immunization. in this paper, the prevalence, control measure, and tolerance of tanzania shorthorn zebu (tshz) cattle to ecf are reviewed. in addition, the opportunities available for reducing the use of acaricides for the benefit of the farmers in terms of reduction of costs of purchasing acaricides ...201728332070
genetic and antigenic diversity of theileria parva in cattle in eastern and southern zones of tanzania. a study to support control of east coast fever.this study investigated the genetic and antigenic diversity of theileria parva in cattle from the eastern and southern zones of tanzania. thirty-nine (62%) positive samples were genotyped using 14 mini- and microsatellite markers with coverage of all four t. parva chromosomes. wright's f index (f(st) = 0 × 094) indicated a high level of panmixis. linkage equilibrium was observed in the two zones studied, suggesting existence of a panmyctic population. in addition, sequence analysis of cd8+ t-cel ...201525417727
loop-mediated isothermal amplification (lamp) assays for detection of theileria parva infections targeting the pim and p150 genes.we have developed two loop-mediated isothermal amplification (lamp) assays for the detection of theileria parva, the causative agent of east coast fever (ecf), an economically important cattle disease in eastern, central and southern africa. these assays target the polymorphic immunodominant molecule (pim) and p150 lamp genes. the primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of t. parv ...201019654009
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