| nested pcr to detect and distinguish the sympatric filarial species onchocerca volvulus, mansonella ozzardi and mansonella perstans in the amazon region. | we present filaria-nested polymerase chain reaction (pcr), which is based on amplification of first internal transcribed spacer rdna to distinguish three parasitic filarial species (onchocerca volvulus, mansonella ozzardi and mansonella perstans) that can be found in the amazon region. nested pcr-based identifications yielded the same results as those utilizing morphological characters. nested pcr is highly sensitive and specific and it detects low-level infections in both humans and vectors. no ... | 2010 | 20945000 |
| atypical mansonella ozzardi microfilariae from an endemic area of brazilian amazonia. | mansonellosis is endemic in several regions of africa, the caribbean, and latin america. mansonella ozzardi and mansonella perstans have been reported in latin america, including the amazon region. a morphological and molecular microfilariae study was performed in pauini (brazil). blood samples were collected from 40 individuals, and were analyzed by giemsa-stained blood film and by two different nested polymerase chain reactions which detect internal transcribed spacer-1 and the major sperm pro ... | 2016 | 27402517 |