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prevalence of pathogenic bacteria in ixodes ricinus ticks in central bohemia.bacteria associated with the tick ixodes ricinus were assessed in specimens unattached or attached to the skin of cats, dogs and humans, collected in the czech republic. the bacteria were detected by pcr in 97 of 142 pooled samples including 204 ticks, i.e. 1-7 ticks per sample, collected at the same time from one host. a fragment of the bacterial 16s rrna gene was amplified, cloned and sequenced from 32 randomly selected samples. the most frequent sequences were those related to candidatus midi ...201626612395
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