| high occurrence of acanthamoeba genotype t4 in soil sources from bolívar state, venezuela. | pathogenic strains of acanthamoeba are causative agents of keratitis and encephalitis that often may end fatal in humans and other animals. in the present study, twenty-seven soil samples were collected in the bolivar state in venezuela and checked for the presence of acanthamoeba. samples were cultivated onto 2% non-nutrient agar plates seeded with a layer of heat killed e. coli. amplification by pcr and sequencing of the df3 region of the 18s rdna of acanthamoeba was carried out in order to co ... | 2016 | 27447209 |