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evaluation of two real-time polymerase chain reaction assays for porcine epidemic diarrhea virus (pedv) to assess pedv transmission in growing pigs.in april 2013, a porcine epidemic diarrhea virus (pedv) epidemic began in the united states. as part of the response, real-time reverse transcription polymerase chain reaction (rt-pcr) assays to detect pedv were developed by several veterinary diagnostic laboratories. our study evaluated rt-pcr pedv assays that detect the n gene (gn) and s gene (gs) for their ability to detect pedv infection and the transmission potential of pigs experimentally exposed to pedv. detection limits and quantificatio ...201626699519
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