| pcr assay detects mannheimia haemolytica in culture-negative pneumonic lung tissues of bighorn sheep (ovis canadensis) from outbreaks in the western usa, 2009-2010. | mannheimia haemolytica consistently causes severe bronchopneumonia and rapid death of bighorn sheep (ovis canadensis) under experimental conditions. however, bibersteinia trehalosi and pasteurella multocida have been isolated from pneumonic bighorn lung tissues more frequently than m. haemolytica by culture-based methods. we hypothesized that assays more sensitive than culture would detect m. haemolytica in pneumonic lung tissues more accurately. therefore, our first objective was to develop a p ... | 2014 | 24171569 |