| cloned cdna of a/swine/iowa/15/1930 internal genes as a candidate backbone for reverse genetics vaccine against influenza a viruses. | reverse genetics viruses for influenza vaccine production usually utilize the internal genes of the egg-adapted a/puerto rico/8/34 (pr8) strain. this egg-adapted strain provides high production yield in embryonated eggs but does not necessarily give the best yield in mammalian cell culture. in order to generate a reverse genetics viral backbone that is well-adapted to high growth in mammalian cell culture, a swine influenza isolate a/swine/iowa/15/30 (h1n1) (rg1930) that was shown to give high y ... | 2012 | 22230579 |
| case series of turkey farms from the h5n2 highly pathogenic avian influenza outbreak in the united states during 2015. | between december 2014 and june 2015, an outbreak of h5n2 hpai caused the largest and most expensive agriculture emergency in u.s. department of agriculture-animal and plant health inspection service history. the outbreak affected 21 states; 232 poultry farms (211 commercial and 21 backyard) were affected, and approximately 49.6 million birds were depopulated on poultry farms. the majority of affected farms were commercial turkey operations (n = 160). this report is a case series describing 104 h ... | 2016 | 27309289 |
| outbreak of h5n2 highly pathogenic avian influenza a virus infection in two commercial layer facilities: lesions and viral antigen distribution. | the largest outbreak of highly pathogenic avian influenza a virus (hpaiv) infection in u.s. history began in december 2014 resulting in the euthanasia of millions of birds and collateral economic consequences to the u.s. poultry industry. we describe 2 cases of h5n2 hpaiv infection in laying hens in iowa. following a sharp increase in mortality with minimal clinical signs, 15 dead birds, from 2 unrelated farms, were submitted to the iowa state university veterinary diagnostic laboratory. common ... | 2016 | 27423731 |
| factors associated with highly pathogenic avian influenza h5n2 infection on table-egg layer farms in the midwestern united states, 2015. | a case-control study was conducted among commercial table-egg layer and pullet operations in iowa and nebraska, united states, to investigate potential risk factors for infection with highly pathogenic avian influenza (hpai) h5n2. a questionnaire was developed and administered to 28 case farms and 31 control farms. data were collected at the farm and barn levels, enabling two separate analyses to be performed-the first a farm-level comparison of case farms vs. control farms, and the second a bar ... | 2016 | 27309288 |