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development and performance evaluation of a streamlined method for nucleic acid purification, denaturation, and multiplex detection of bluetongue virus and epizootic hemorrhagic disease virus.bluetongue virus (btv) and epizootic hemorrhagic disease virus (ehdv) possess similar structural and molecular features, are transmitted by biting midges (genus culicoides), and cause similar diseases in some susceptible ruminants. generally, btv causes subclinical disease in cattle, characterized by a prolonged viremia. ehdv-associated disease in cattle is less prominent; however, it has emerged as a major economic threat to the white-tailed deer (odocoileus virginianus) industry in many areas ...201324091683
characterization of epizootic hemorrhagic disease virus from a bovine with clinical disease with high nucleotide sequence identity to white-tailed deer isolates.epizootic hemorrhagic disease virus (ehdv) was isolated from a pregnant cow in indiana, usa, exhibiting excessive salivation, pyrexia and abortion. vp2, vp5, and vp7 sequences of the isolated bovine ehdv showed 97.7, 97.4, and 97.9 % identity to a serotype 2 reference virus. bovine ehdv was closely related (>99.9 %) to white tailed deer (wtd) ehdv collected from iowa in 2013 and showed less than 2.1 % divergence from ehdv collected from wtd across the usa in 2013. the high degree of sequence ide ...201424852073
the first 10 years (2006-15) of epizootic hemorrhagic disease virus serotype 6 in the usa.epizootic hemorrhagic disease virus (ehdv) is a culicoides biting midge-transmitted orbivirus (family reoviridae) of wild and domestic ruminants and is an important pathogen of white-tailed deer (odocoileus virginianus). historically, only two serotypes, ehdv-1 and ehdv-2, have been known to be endemic in the us. however, in 2006, an exotic serotype (ehdv-6) was first detected in the united states by a long-term passive surveillance system for ehdv and bluetongue viruses. here we report ehdv-6 d ...201728657859
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