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molecular characterization of pyraclostrobin resistance and structural diversity of the cytochrome b gene in botrytis cinerea from apple.botrytis cinerea isolates obtained from apple orchards were screened for resistance to the quinone outside inhibitor (qoi) pyraclostrobin. of the 220 isolates tested, 43 (19.5%) were resistant to pyraclostrobin. analysis of partial sequences of the cytochrome b gene (cyt b) in five pyraclostrobin-resistant (pr) and five pyraclostrobin-sensitive (ps) isolates showed that pr isolates harbored the point mutation leading to the substitution of glycine by alanine at codon position 143 in cyt b (g143a ...201122085296
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