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genetic analysis of a novel alaska barley yellow dwarf virus in the family luteoviridae.a new plant virus belonging to the family luteoviridae and isolated from diseased oat (avena sativa l.) plants was discovered in alaska in 2003. even though plants with red/orange leaves were indicative of barley yellow dwarf disease, they were not reactive to specific antibodies corresponding to barley yellow dwarf virus (bydv)-mav, -pav, -sgv, and cereal yellow dwarf virus-rpv from enzyme-linked immunosorbent assays (elisa). an alternative rt-pcr assay that incorporated shu-f/yan-r primers for ...200717013543
genetic structure in natural populations of barley/cereal yellow dwarf virus isolates from alaska.the genetic structure of natural populations of alaskan barley yellow dwarf virus (bydv)-pav, bydv-pas, and cereal yellow dwarf virus (cydv)-rpv from barley (hordeum vulgare l.) and oats (avena sativa l.) in alaska were analyzed between 2002 and 2004. pcr products spanning the viral coat protein gene of 187 isolates were cloned and sequenced. the majority (78%) were similar to bydv-pas, 19% were similar to cydv-rpv, and only about 3% resembled bydv-pav. the cydv-rpv isolates clustered in three g ...200717216137
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