quantification of rhvt-f genome load in feather follicles by specific real-time qpcr as an indicator of ndv-specific humoral immunity induced by day-old vaccination in spf chickens.the purpose of this study was to look for a reliable molecular method for confirmation of uptake of recombinant turkey herpesvirus vaccine against newcastle disease (rhvt-f) and for use as a valuable prediction tool of newcastle disease virus (ndv)-specific immune response in chickens deprived of maternally derived antibody (mda). a quantitative real-time polymerase chain reaction (real-time qpcr) specific to rhvt-f was developed. the method was applied to various tissue samples taken from speci ...201525687165
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