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filariasis in tak province, northwest thailand: the presence of subperiodic variant wuchereria bancrofti.the microfilariae found in carriers at tak province, northwestern thailand were morphologically and morphometrically studied. it was found that the parasites conformed to that of w. bancrofti microfilaria. the microfilarial periodicity as determined from four carriers was found to be nocturnally (early evening) subperiodic type showing a distinct peak at 1800 hours.19873313738
a polymerase chain reaction assay for the survey of bancroftian filariasis.a polymerase chain reaction (pcr) assay based on a highly repeated dna sequence found in wuchereria bancrofti (sspi repeat) has been modified for the survey of bancroftian filariasis in expatriate workers (myamese, karen and mon) from myanmar where human filariasis is endemic. the pcr was very sensitive with the ability to detect the presence of as little as 10 pg of parasite dna. the primers used in this pcr also showed highly specific amplification of parasite dna without the presence of non-s ...200212693583
comparative assessment of an og4c3 elisa and an ict filariasis test: a study of myanmar migrants in thailand.detection of circulating filarial antigen has now emerged as an alternative method for the diagnosis of bancroftian filariasis. we compared two antigen detection assays, an og4c3 elisa and an ict (immunochromatography) filariasis test, for the diagnosis of wuchereria bancrofti infections in migrant myanmar workers in tak province, western thailand. a total of 337 myanmars participated in this study. the microfilarial rate was 3.3%. the og4c3 elisa could detect 19.1% of bancroftian filariasis whi ...200315198343
culex quinquefasciatus in phitsanulok as a possible vector of nocturnally periodic wuchereria bancrofti transmission in myanmar immigrants.the present study was undertaken in order to study whether culex quinquefasciatus collected in phitsanulok province can be an insect host for the development of wuchereria bancrofti larvae. w. bancrofti infected blood from myanmar workers in mae sot, tak province was fed to mosquitoes by using the artificial membrane feeding. an infection of w. bancrofti was found with the highest density of l3 in the mosquito thorax on the 14th day after feeding. the infection rate also correlated to the densit ...200516438205
diversity of anopheles species and trophic behavior of putative malaria vectors in two malaria endemic areas of northwestern thailand.we determined the species diversity, blood-feeding behavior, and host preference of anopheles mosquitoes in two malaria endemic areas of tak (mae sot district) and mae hong son (sop moei district) provinces, located along the thai border with myanmar, during a consecutive two-year period. anopheline mosquitoes were collected using indoor and outdoor human-landing captures and outdoor cow-baited collections. mosquitoes were initially identified using morphological characters, followed by the appr ...201425424272
relationship between male hydrocele and infection prevalences in clustered communities with uncertain transmission of wuchereria bancrofti on the thailand-myanmar border.a cross-sectional community-based study was conducted in three clustered communities, belonging to a single small village in mae chan subdistrict, umphang district, tak province, close to the thailand-myanmar border, where regular night blood survey have been discontinued since 1997 and no epidemiological study had been conducted. in order to understand prevalences of distribution of male hydrocele and infection in clinically diagnostic and epidemiologic implications in uncertain transmission of ...200212118464
endemic bancroftian filariasis in thailand: detection by og4c3 antigen capture elisa and the polymerase chain reaction.lymphatic filariasis, mainly caused by wuchereria bancrofti and brugia malayi, has been targeted for elimination by the world health organization by the year 2020. to achieve this goal, highly sensitive and specific diagnostic tests are necessary for close monitoring and evaluation of the control program. we employed an elisa to detect the og4c3 antigen and a polymerase chain reaction-based assay for diagnosis of w. bancrofti infection, among the thai-karen population in tak province, thailand. ...200111800304
detection of plasmodium falciparum and wuchereria bancrofti infected blood samples using multiplex pcr.a rapid and sensitive multiplex pcr has been developed for the diagnosis of multiple parasitic infection in human blood. infection is detected by a single multiplex pcr reaction containing two pairs of oligonucleotide primers whereby each primer is specific for each parasite species. these primer sets amplified 400 and 450-bp fragments for wuchereria bancrofti and 208-bp fragment for plasmodium falciparum. the pcr products derived from each parasite species were visualized in ethidium bromide-st ...200111513554
field trial of the ict filariasis for diagnosis of wuchereria bancrofti infections in an endemic population of thailand.the ict filariasis, a rapid card test format, which is based on qualitative detection by monoclonal antibody of the circulating antigen of wuchereria bancrofti adult worm, is a new diagnostic test of choice for determining the infections under field conditions. by using clinical and recall techniques and microscopy (thick smear and capillary tube technique) as reference, we assessed the efficiency of the ict card test in sera from 225 subjects living in w. bancrofti-endemic villages of tak provi ...199910774670
association between toll-like receptor 2 (tlr2) polymorphisms and asymptomatic bancroftian filariasis.lymphatic filariasis is mainly caused by the filarial nematodes wuchereria bancrofti and brugia malayi. wolbachia, intracellular symbiotic bacteria in filarial parasite, is known to induce immune response predominantly through toll-like receptor 2 (tlr2). this study was performed to investigate the association between polymorphisms of the tlr2 gene and susceptibility to asymptomatic bancroftian filariasis. a total of 142 unrelated asymptomatic bancroftian filariasis patients and 151 endemic norm ...201020549240
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