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molecular characterization of vp4, vp6, vp7, nsp4, and nsp5/6 genes identifies an unusual g3p[10] human rotavirus strain.an unusual strain of human rotavirus g3p[10] (cmh079/05) was detected in a stool sample of a 2-year-old child admitted to the hospital with severe diarrhea in chiang mai, thailand. analysis of the vp7 gene sequence revealed highest identities with unusual human rotavirus g3 strain cmh222 at 98.7% on the nucleotide and 99.6% on the amino acid levels. phylogenetic analysis of the vp7 sequence confirmed that the cmh079/05 strain formed a cluster with g3 rotavirus reference strains and showed the cl ...200919031442
genetic characterization of group c rotavirus isolated from a child hospitalized with acute gastroenteritis in chiang mai, thailand.during an epidemiological survey of human rotavirus infection in chiang mai, thailand, from 2002 to 2004, in which 263 stool specimens tested, one isolate of group c rotavirus was detected from a two-year-old child admitted to hospital with acute gastroenteritis. the human group c rotavirus, named cmh004/03, was characterized further by molecular analyses of its vp4, vp6, and vp7 gene segments as well as determination of rna pattern by polyacrylamide gel electrophoresis (page). molecular charact ...200818696224
molecular characterization of a rare g3p[3] human rotavirus reassortant strain reveals evidence for multiple human-animal interspecies transmissions.an unusual strain of human rotavirus g3p[3] (cmh222), bearing simian-like vp7 and caprine-like vp4 genes, was isolated from a 2-year-old child patient during the epidemiological survey of rotavirus in chiang mai, thailand in 2000-2001. the rotavirus strain was characterized by molecular analysis of its vp4, vp6, vp7, and nsp4 gene segments. the vp4 sequence of cmh222 shared the greatest homology with those of caprine p[3] (grv strain) at 90.6% nucleotide and 96.4% amino acid sequence identities. ...200616721863
antigenic and genetic analyses of human rotaviruses in chiang mai, thailand: evidence for a close relationship between human and animal rotaviruses.serotyping of group a rotaviruses obtained from stools of 158 diarrheic patients in chiang mai, thailand, by elisa with monoclonal antibodies revealed a yearly change in the prevalence of individual serotypes. three unusual human rotavirus strains were isolated. strain mc35 had subgroup i-serotype 10 antigen and a long rna electrophoretic type, a property hitherto found only in bovine rotaviruses. rna-rna hybridization tests showed that the strain is more closely related genetically to bovine th ...19921378870
molecular characterization of rare g3p[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis.in 2004, an epidemiological survey of human rotavirus infection in chiang mai, thailand detected two uncommon human rotavirus strains (cmh120/04 and cmh134/04) bearing au-1-like g3p[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. the cmh120/04 and cmh134/04 rotavirus strains were characterized by molecular analyses of their vp6, vp7, vp8*, and nsp4 gene segments as well as the determination of rna patterns by polyacrylamide gel electrophoresis (page). analysis of the ...200717457907
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