| a new pentaplex-nested pcr to detect five pathogenic bacteria in free living amoebae. | changes in water use and anthropogenic activity have major impacts on the quality of natural aquatic ecosystems, water distribution and wastewater plants. one of the main problems is the presence of some pathogenic microorganisms that are resistant to disinfection procedures when they are hosted by free living amoeba and that in many cases are hardly detectable by culture-based procedures. in this work we report a sensitive, low-cost procedure consisting of a pentaplex-nested pcr that allows sim ... | 2013 | 23168310 |