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application of real-time pcr for the detection of strongyloides spp. in clinical samples in a reference center in spain.strongyloidiasis is one of the major intestinal helminthic infections in humans with a worldwide distribution, affecting especially tropical and subtropical regions. this disease can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection. definitive diagnosis of strongyloides stercoralis infection relies mainly on demonstration of larvae in stool, but at present there is no gold standard for this diagnosis. our main objective was to evaluate a real-time pcr ...201525447829
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