development of a duplex rapid assay for immunoglobulins m and g to evaluate the parvoviral immune status of clinically healthy dogs.a duplex rapid assay for detection of serum antibodies to canine parvovirus (cpv) was developed. canine immunoglobulin (ig)m or igg were captured in immunotubes with anti-canine igm or igg and detected with parvovirus vp2 recombinant protein followed by an anti-vp2 monoclonal antibody. the assay was tested using a collection of sera from dogs that were vaccinated against cpv on arrival at an animal shelter in madrid, spain. results were compared with those of 2 commercial enzyme-linked immunosor ...201626951330
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