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high prevalence of subtype 4 among isolates of blastocystis hominis from symptomatic patients of a health district of valencia (spain).in order to know the genetic diversity of blastocystis hominis from a health district of valencia (spain) 51 clinical isolates from symptomatic patients, 31 axenic and 20 monoxenic, were ribotyped by analysing the restriction fragment length polymorphism (rflp) of amplicons obtained by polymerase chain reaction (pcr) of small-subunit of ribosomal dna genes (ssu-rdna). for this purpose, dna was subjected to two independent pcr (rd3-rd5, f1-r1) and to three independent treatments with restrictases ...200919471964
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