use of envelope domain iii protein for detection and differentiation of flaviviruses in the free state province, south africa.the presence of the mosquito-borne flavivirus species west nile virus (wnv) and wesselsbron virus (wessv) in southern africa is well established; however, their true prevalence remains unknown. to date, the presence of tick-borne flaviviruses has not been confirmed in this region. serological assays using reagents that can be handled in a biosafety level 2 or lower facility were developed and evaluated for the detection and differentiation of tick- and mosquito-borne flaviviruses in the free sta ...201424689894
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