| determination of the genetic similarities of fingerprints from escherichia coli o157:h7 isolated from different sources in the north west province, south africa using isr, boxair and rep-pcr analysis. | the aim of this study was to determine the genetic relationships of escherichia coli o157:h7 isolated from pigs, cattle, pork, beef, humans and water samples using rep, isr and boxair pcr analysis. a total of 94 isolates were subjected to the rep-pcr analysis while 95 were screened for isr and boxair pcr fingerprints. the band sizes for amplicons from the isr-pcr analysis ranged from 0.173kb to 0.878kb. however, a large proportion of the isolates had four bands ranging from 0.447kb to 0.878kb. c ... | 2013 | 23557962 |
| genotypic characterization of escherichia coli o157:h7 isolates from different sources in the north-west province, south africa, using enterobacterial repetitive intergenic consensus pcr analysis. | in many developing countries, proper hygiene is not strictly implemented when animals are slaughtered and meat products become contaminated. contaminated meat may contain escherichia coli (e. coli) o157:h7 that could cause diseases in humans if these food products are consumed undercooked. in the present study, a total of 94 confirmed e. coli o157:h7 isolates were subjected to the enterobacterial repetitive intergenic consensus (eric) polymerase chain reaction (pcr) typing to generate genetic fi ... | 2014 | 24886815 |