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characterization of lettuce big-vein associated virus and mirafiori lettuce big-vein virus infecting lettuce in saudi arabia.during 2014 and 2015, 97 lettuce plants that showed big-vein-disease-like symptoms and seven weed plants were collected from the riyadh region. das-elisa revealed that 25% and 9% of the lettuce plants were singly infected with lbvav and milbvv, respectively, whereas 63% had a mixed infection with both viruses. the results were confirmed by multiplex reverse transcription polymerase chain reaction using primers specific for lbvav and milbvv. lbvav and milbvv were also detected in sonchus oleraceu ...201728299485
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