| simultaneous detection of both rna and dna viruses infecting dry bean and occurrence of mixed infections by bgymv, bcmv and bcmnv in the central-west region of mexico. | a multiplex reverse transcription polymerase chain reaction (rt-pcr) assay was developed to simultaneously detect bean common mosaic virus (bcmv), bean common mosaic necrotic virus (bcmnv), and bean golden yellow mosaic virus (bgymv) from common bean leaves dried with silica gel using a single total nucleic acid extraction cetyl trimethyl ammonium bromide (ctab) method. a mixture of five specific primers was used to amplify three distinct fragments corresponding to 272 bp from the ac1 gene of bg ... | 2017 | 28358318 |