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isolation of a megatrypanum trypanosome from sika deer (cervus nippon yesoensis) in japan.a trypanosome was isolated from a sika deer (cervus nippon yesoensis) in hokkaido, japan, during the primary culture of sika deer renal cells. this is the first report of isolation of a megatrypanum trypanosome from japanese cervidae. the trypanosome, designated tsd1, was propagated and maintained in eagle's modified essential medium containing 20% fetal bovine serum with sika deer renal cells as feeder. the tsd1 trypanosome was morphometrically similar to trypanosoma cervi, which is commonly is ...200717714873
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