| synthesis of 2-phenylbenzotriazole-type mutagens, pbta-5 and pbta-6, and their detection in river water from japan. | we previously determined the chemical structures of four 2-phenylbenzotriazole mutagens (pbta-1, -2, -3 and -4) in blue rayon-adsorbed material from the nishitakase river in kyoto prefecture and the nikko river in aichi prefecture in japan. on the basis of a synthesis study, these four pbta derivatives were deduced to have originated from corresponding dinitrophenylazo dyes by reduction and chlorination. 2-[(2-bromo-4,6-dinitrophenyl)azo]-5-[bis(2-acetoxyethyl) amino]-4-methoxyacetanilide (color ... | 2001 | 11673076 |
| isolation and identification of a new 2-phenylbenzotriazole-type mutagen (pbta-3) in the nikko river in aichi, japan. | we have previously determined the chemical structures of two 2-phenylbenzotriazole mutagens (pbta-1 and pbta-2) in blue cotton-adsorbed material from the nishitakase river in kyoto, japan. in the present study, further analysis of mutagenic substances in the nikko river, which flows through aichi prefecture in japan, allowed the isolation of a new mutagen. material (2.2 g) adsorbed on blue cotton (3 kg) at a site below the sewage plant on the nikko river was purified by various column chromatogr ... | 2000 | 10898584 |
| identification of a 2-phenylbenzotriazole (pbta)-type mutagen, pbta-2, in water from the nishitakase river in kyoto. | we previously isolated five mutagens, compounds i-v, in blue rayon-adsorbed materials from the nishitakase river in kyoto. the chemical structure of compound i, a major mutagen that accounted for 21% of the total mutagenicity, was determined to be 2-[2-(acetylamino)-4-[bis(2-methoxyethyl)amino]-5-methoxyphenyl]-5-am ino-7-bromo-4-chloro-2h-benzotriazole (pbta-1). compound ii was also a major mutagen and accounted for 17% of the total mutagenicity. in this study, a large quantity (1.2 mg) of comp ... | 1998 | 9778316 |
| isolation and chemical-structural determination of a novel aromatic amine mutagen in water from the nishitakase river in kyoto. | water samples from the nishitakase river in kyoto, japan, especially taken at sites below sewage plants, show significantly high mutagenicity in the ames test. in the present study, mutagens in the river water were adsorbed to 24 g of blue rayon, extracted, and separated by hplc on ods columns. five mutagenic compounds (i-v) were isolated, and they accounted for 21%, 17%, 11%, 12%, and 6%, respectively, of the total mutagenicity of the blue rayon-adsorbed materials. with compound i obtained from ... | 1997 | 9348426 |
| evaluation of blue-chitin column, blue-rayon hanging, and xad-resin column techniques for concentrating mutagens from two japanese rivers. | highly mutagenic water of the katsura river, kyoto, and moderately mutagenic water of the asahi river, okayama, were used to evaluate the efficacy of three concentration techniques, the blue-chitin column, the blue-rayon hanging, and the xad-2 column. these two river waters have been shown to exhibit high mutagenicity in the assay with salmonella typhimurium ta98 with metabolic activation. with this assay as a measure, two water samples from the katsura, collected on different dates, and a sampl ... | 1996 | 8950353 |
| isolation of mutagenic components by high-performance liquid chromatography from xad extract of water from the nishitakase river, kyoto city, japan. | in order to determine the concentrations of mutagenic pollutants in water samples taken from the nishitakase river in kyoto city, japan, the organic fraction was concentrated by xad adsorption followed by elution with ethyl ether. salmonella typhimurium tester strains ta1538 and ta98 were used fro detecting mutagenic activity. mutagenic fractions, separated by a liquid-liquid fractionation and two consecutive runs with thin layer chromatography (tlc), were analysed using high-performance liquid ... | 1986 | 3810145 |
| chemical synthesis of a novel aromatic amine mutagen isolated from water of the nishitakase river in kyoto and a possible route of its formation. | among five mutagenic compounds isolated from water samples, taken at sites below the sewage plants of the nishitakase river in kyoto, japan, the structure of compound i has been determined to be 2-[2-(acetylamino)-4-[bis(2-methoxyethyl)amino]-5-methoxyphenyl]-5-am ino-7-bromo-4-chloro-2h-benzotriazole (pbta-1). since this novel aromatic amine mutagen has characteristic substituents in its molecule, it is postulated that the azo dye, 2-[(2-bromo-4, 6-dinitrophenyl)azo]-4-methoxy-5-[bis(2-methoxye ... | 1998 | 9548809 |
| isolation and identification of non-chlorinated phenylbenzotriazole (non-clpbta)-type mutagens in the ho river in shizuoka prefecture, japan. | we previously identified 2-[2-(acetylamino)-4-amino-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2h-benzotriazole (pbta) congeners as major mutagens in water concentrates from several rivers that flow in three different areas, i.e. kyoto, aichi, and fukui prefectures, in japan. in synthesis studies, these pbtas were shown to be formed from corresponding dinitrophenylazo dyes via non-chlorinated derivatives (non-clpbtas). however, only non-clpbta-1, i.e. 2-[2-(acetylamino)-4-[bis(2-methoxyethyl)amin ... | 2006 | 16962368 |
| mutagenicity in salmonella typhimurium tester strains of xad-2-ether extract, recovered from katsura river water in kyoto city, and its fractions. | the mutagenic activity of xad-2-ether extracts recovered from katsura river water at monthly intervals during september to december 1980 was tested on s. typhimurium ta1538, ta1535, ta98 and ta100. the extracts showed strong mutagenic activity towards ta1538 nd ta98, especially in the presence of s9 mix. they were more active to ta1538 than to ta98. some of each of the xad-2-ether extracts were pooled and separated into neutral, basic and acidic fractions, and their mutagenic activities were tes ... | 1982 | 6750370 |