| prevalence and risk factor analysis of bovine hemoplasma infection by direct pcr in eastern hokkaido, japan. | the prevalence and risk factors of 2 bovine hemoplasma species-mycoplasma wenyonii (mw) and "candidatus mycoplasma haemobos" (cmh)-were examined by direct pcr using whole blood samples collected from 343 cattle in eastern hokkaido, japan. the sensitivity of the direct pcr assay was 10-fold higher than the standard pcr assay, detecting 5 copies of bovine hemoplasma dna per reaction. an epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was perfo ... | 2012 | 22673725 |