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diversity of 16s ribosomal dna-defined bacterial population in acid rock drainage from japanese pyrite mine.four acidophilic bacteria (yards1-4) were isolated from an acid rock drainage (ard) from yanahara mine, okayama prefecture, japan. the physiological and 16s rdna sequence analyses revealed that yard1 was closely affiliated with acidithiobacillus ferrooxidans, yard2 was an acidiphilium-like bacterium, and yard3 and yard4 were sulfur-oxidizing bacteria with a relatively close relationship to a. ferrooxidans in the phylogenetic analysis. a molecular approach based on the construction of a 16s rdna ...200516473774
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