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use of pcr-restriction fragment length polymorphism analysis for identification of yeast species isolated from bovine intramammary infection.this study reports a rapid pcr-based technique using a one-enzyme rflp for discrimination of yeasts isolated from bovine clinical and subclinical mastitis milk samples. we analyzed a total of 1,486 milk samples collected over 1 yr in south sardinia and northern italy, and 142 yeast strains were preliminarily grouped based on their cultural morphology and physiological characteristics. assimilation tests were conducted using the identification kit api id 32c and apilab plus software (biomérieux, ...201324119798
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