strain-specific scar markers for the detection of trichoderma harzianum as12-2, a biological control agent against rhizoctonia solani, the causal agent of rice sheath order to identify a specific marker for t. harzianum as12-2, a strain capable of controlling rice sheath blight caused by rhizoctonia solani, up-pcr was performed using five universal primers (up) both separately and in pairwise combinations. the application of two up primers resulted in the amplification of unique fragments from the genomic dna of t. harzianum as12-2, clearly distinguishing it from other trichoderma strains. the unique fragments had no significant sequence homology with any ...201121388921
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