genotype identification of human cystic echinococcosis in isfahan, central iran.echinococcosis/hydatidosis is one of the most important zoonotic diseases commonly found in different regions of iran with a major economic and public health importance. in the current study, echinococcus granulosus isolates were collected from hospitalized patients in isfahan, central iran. the genotypes of 30 samples were determined by polymerase chain reaction amplification of internal transcribed spacer-1 region of ribosomal dna, followed by restriction fragment length polymorphism (rflp) wi ...201020549235
genetic diversity of echinococcus granulosus in center of iran.hydatid cyst caused by echinococcus granulosus is one of the most important parasitic diseases around the world and many countries in asia, including iran, are involved with this infection. this disease can cause high mortality in humans as well as economic losses in livestock. to date, several molecular methods have been used to determine the genetic diversity of e. granulosus. so far, identification of e. granulosus using real-time pcr fluorescence-based quantitative assays has not been studie ...201425246720
hydatidosis in camels (camelus dromedarius) and their potential role in the epidemiology of echinococcus granulosus in iran.hydatid cysts were recovered from 35.2% (233/661) of camels (camelus dromedarius) slaughtered in five different regions of iran. the degree of prevalence between males (34.4%) and females (36.6%) was not statistically significant. the highest rate of infection (59.3%) was found in the isfahan region (in the central part of iran) while the lowest (25.7%) was found in kerman province. the organ distribution of cysts was 49.4% in lungs alone, 30.0% in both liver and lungs, 14.6% in liver only and 6 ...200515946390
rapid identification of echinococcus granulosus and e. canadensis using high-resolution melting (hrm) analysis by focusing on a single nucleotide polymorphism.high-resolution melting (hrm) is a reliable and sensitive scanning method to detect variation in dna sequences. we used this method to better understand the epidemiology and transmission of echinococcus granulosus. we tested the use of hrm to discriminate the genotypes of e. granulosus and e. canadensis. one hundred forty-one hydatid cysts were collected from slaughtered animals in different parts of isfahan-iran in 2013. after dna extraction, the mitochondrial cytochrome c oxidase subunit 1 (co ...201626567833
molecular characterization of human and animal echinococcus granulosus isolates in isfahan, iran.cystic hydatid disease (chd) is one of the most important zoonotic diseases in different parts of iran. while it causes major health problem, there is limited information about its transmission cycles and reservoirs of human infection. therefore we aimed to characterize the existence echinococcus granulosus cysts in humans and animals in the province of isfahan, central region of iran. we collected hydatid cysts from the liver and lungs of patients who underwent surgery procedure and also cysts ...201120858453
echinococcus granulosus infection of farm dogs of iran.the prevalence and distribution of echinococcus granulosus in sheepdogs was studied in 13 provinces of iran, where 90% of the iranian sheep and goat populations and, thus, sheepdogs are found. worms were found in 27.17% of 390 dogs successfully purged with 4 mg/kg arecoline hydrobromide. the highest prevalence was detected in dogs from the rural areas of isfahan (central part of iran) and the lowest, in dogs from those of sistan (southeast iran). the frequency distribution of e. granulosus was o ...19989521009
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