| molecular detection of brucella species in patients suspicious of brucellosis from zanjan, iran. | brucella is an intracellular pathogen capable of infecting animals and humans. the aim of this study was to identify brucella spp in sera of high risk individuals by a polymerase chain reaction (pcr)-based method. a total of 180 patients suspected to have brucellosis were examined by serological tests. to establish a pcr protocol for diagnosis of active brucellosis, dna was extracted from the serum samples by using a commercial kit. pcr amplification was done for detection of brocella dna using ... | 2014 | 25242938 |