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serodiagnosis of toxoplasma gondii infection in bovines from kerala, india using a recombinant surface antigen 1 elisa.data on the prevalence of toxoplasmosis in farm animals from india is scanty. though a few reports exist on prevalence of toxoplasmosis in small ruminants, information on toxoplasmosis in large ruminants is virtually nonexistent from india. an antibody detection recombinant elisa specific for toxoplasma gondii was laboratory standardized using recombinant surface antigen 1 (sag1) protein. a 958-bp truncated sequence coding for tachyzoite stage specific sag1 protein was amplified and expressed in ...201525952097
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