| rapid and sensitive detection of dasheen mosaic virus infecting elephant foot yam by reverse transcription loop mediated isothermal amplification of coat protein gene. | dasheen mosaic virus (dsmv), the pathogen causing mosaic disease of elephant foot yam (amorphophallus paeoniifoilius) is disseminated mainly through vegetative propagation of the tubers. for the rapid and sensitive detection of the virus, a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay based on the coat protein gene has been developed. a final concentration of 5.4 mm magnesium sulphate and 0.7 m betaine in the reaction mixture was found to be optimum for getting ch ... | 2015 | 26096915 |