| isolation and characterization of novel lipase gene liphim1 from the dna isolated from soil samples. | metagenomics is a magnificent tool to isolate genes from unknown/uncharacterized species and also from organisms that cannot be cultured. in this study, we constructed a metagenomic library from isolated dna of soil samples collected from palamuru university campus premises, in mahabubnagar district of andhra pradesh, india. we isolated a novel lipase gene liphim1, which has an open reading frame of 591 base pairs and encodes ∼23 kda protein consisting of 196 amino acids. the lipase liphim1 show ... | 2014 | 24723100 |