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clinical validation of 3 commercial real-time reverse transcriptase polymerase chain reaction assays for the detection of middle east respiratory syndrome coronavirus from upper respiratory tract specimens.since discovery of middle east respiratory syndrome coronavirus (mers-cov), a novel betacoronavirus first isolated and characterized in 2012, mers-cov real-time reverse transcriptase polymerase chain reaction (rrt-pcr) assays represent one of the most rapidly expanding commercial tests. however, in the absence of extensive evaluations of these assays on positive clinical material of different sources, evaluating their diagnostic effectiveness remains challenging. we describe the diagnostic perfo ...201728104327
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