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isolation and differentiation of methanogenic archaea from mesophilic corn-fed on-farm biogas plants with special emphasis on the genus methanobacterium.in this study, methanogenic archaea were isolated from five full-scale agricultural biogas plants (bgps) located in rhineland-palatinate and saarland, germany, digesting maize silage and cattle manure. according to partial 16s rrna gene sequences, the strains isolated from enrichment cultures were related to methanoculleus bourgensis, methanosarcina mazei, methanosaeta concilii, and methanobacterium formicicum. the 16s rrna gene libraries of two representative bgps screened with the direct ampli ...201424639207
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