| a novel virus of the genus cilevirus causing symptoms similar to citrus leprosis. | citrus leprosis in colombia was previously shown to be caused by cytoplasmic citrus leprosis virus (cilv-c). in 2011, enzyme-linked immunosorbent assay and reverse-transcription polymerase chain reaction (rt-pcr)-based diagnostic methods failed to identify cilv-c from citrus samples with symptoms similar to citrus leprosis; however, virions similar to cilv-c were observed in the cytoplasm of the symptomatic leaves by transmission electron microscopy. furthermore, the causal organism was transmit ... | 2013 | 23268581 |
| production of mono- and polyclonal antibodies to citrus leprosis virus c2 and their application in triple antibody sandwich elisa and immunocapture rt-pcr diagnostic assays. | the newly discovered citrus leprosis virus cytoplasmic type 2 (cilv-c2) is one of the causal virus of citrus leprosis disease complex; which leads to substantial loss of citrus production in the states of meta and casanare of colombia. specific and sensitive detection methods are needed to monitor the dissemination of cilv-c2 in colombia, and to prevent introduction of cilv-c2 to other citrus growing countries. toward this end, putative coat protein gene (cpg) of cilv-c2 was amplified from cilv- ... | 2017 | 28237659 |