| characterization of vibrio cholerae isolates from 1976 to 2013 in shandong province, china. | cholera continues to be a serious public health issue in developing countries. we analyzed the epidemiological data of cholera from 1976 to 2013 in shandong province, an eastern coastal area of china. a total of 250 vibrio cholerae isolates were selected for pcr analysis of virulence genes and pulsed-field gel electrophoresis (pfge). the analysis of the virulence genes showed that the positive rates for tcpa and tcpi were the highest among strains from the southwest region, which had the highest ... | 2017 | 27780663 |