the survey of porcine teschoviruses, porcine circovirus and porcine transmissible gastroenteritis virus infecting piglets in clinical specimens in china.a multiplex pcr assay was developed and evaluated for its ability to simultaneously detect three viral infections of swine. specific primers were carefully selected from articles published for each of the following three viruses: porcine circovirus type ii (pcv2), porcine teschovirus (ptv) and porcine transmissible gastroenteritis virus (tgev). each target produced a specific amplicon with a size of 353 bp (pcv2), 168 bp (ptv) and 499 bp (tgev). the sensitivity of the multiplex pcr using purifie ...201323224864
a recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus.recently, porcine deltacoronavirus (pdcov) has been proven to be associated with enteric disease in piglets. diagnostic tools for serological surveys of pdcov remain in the developmental stage when compared with those for other porcine coronaviruses. in our study, an indirect enzyme-linked immunosorbent assay (elisa) (rpdcov-n-elisa) was developed to detect antibodies against pdcov using a histidine-tagged recombinant nucleocapsid (n) protein as an antigen. the rpdcov-n-elisa did not cross-react ...201626668175
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