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isolation and identification of duck tembusu virus strain lh and development of latex-agglutination diagnostic method for rapid detection of antibodies.summary. an outbreak of egg-drop syndrome occurred on a sheldrake duck farm in longhai in fujian province, china, in 2012. the main clinical symptoms were sharply reduced egg production, crooked necks, and death. we isolated the virus from the sick ducks, identified it, and observed the histopathologic changes after viral infection. we detected viral rna in the blood and feces of the infected ducks and developed a latex-agglutination diagnostic method to detect anti-tembusu-virus antibodies. our ...201425619007
isolation and characterization of a chinese strain of tembusu virus from hy-line brown layers with acute egg-drop syndrome in fujian, china.tembusu virus (tmuv) has been a causative agent of an acute egg-drop syndrome found in chinese duck populations since at least 2010. in this paper, we report the characterization of a tmuv-like flavivirus (named cjd05) isolated from naturally infected egg-laying fowl. the virus was identified and then isolated from hens suffering from severe egg drop and fever in fujian province, china. the virus replicated well in mdef and cef cells, and its cytopathogenic effect (cpe) was apparent. hemagglutin ...201424297489
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