ducks as a potential reservoir for pasteurella multocida infection detected using a new romph-based elisa. | pasteurella multocida is an important pathogen of numerous domestic poultry and wild animals and is associated with a variety of diseases including fowl cholera. the aim of this study was to develop an indirect enzyme-linked immunosorbent assay (elisa) based on recombinant outer-membrane protein h (romph) for detection of anti-p. multocida antibodies in serum to determine their prevalence in chinese ducks. the p. multocida omph gene was cloned into pet32a, and romph was expressed in escherichia ... | 2017 | 28626158 |