detection of papaya leaf distortion mosaic virus by reverse-transcription loop-mediated isothermal amplification. | papaya leaf distortion mosaic virus (pldmv) can infect transgenic papaya resistant to a related pathogen, papaya ringspot virus (prsv), posing a substantial threat to papaya production in china. current detection methods, however, are unable to be used for rapid detection in the field. here, a reverse-transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for the detection of pldmv, using a set of four rt-lamp primers designed based on the conserved sequence of pldmv ... | 2014 | 24100065 |
geographical and genetic divergence among papaya ringspot virus populations within hainan province, china. | papaya ringspot virus (prsv) severely affects the global papaya industry. transgenic papaya has been proven to have effective resistance to prsv isolates from hawaii, thailand, taiwan, and other countries. however, those transgenic cultivars failed to show resistance to hainan island isolates. some 76 prsv samples, representative of all traditional papaya planting areas across five cities (wen chang, n = 13; cheng mai, n = 14; chang jiang, n = 11; le dong, n = 25; and san ya, n = 13) within hain ... | 2016 | 27070425 |