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detection of papaya leaf distortion mosaic virus by reverse-transcription loop-mediated isothermal amplification.papaya leaf distortion mosaic virus (pldmv) can infect transgenic papaya resistant to a related pathogen, papaya ringspot virus (prsv), posing a substantial threat to papaya production in china. current detection methods, however, are unable to be used for rapid detection in the field. here, a reverse-transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for the detection of pldmv, using a set of four rt-lamp primers designed based on the conserved sequence of pldmv ...201424100065
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