| improved duplex rt-pcr assay for differential diagnosis of mixed infection of duck hepatitis a virus type 1 and type 3 in ducklings. | infection with duck hepatitis a virus (dhav) causes an acute, rapidly spreading, and fatal disease of young ducklings. dhav type 1 (dhav-1) and type 3 (dhav-3) have been identified in china. in this study, a duplex rt-pcr assay was developed to identify dhav-1 and dhav-3 with mixed infection. the method was shown to be high specificity and sensitivity. the minimum detection limit of the method has been determined to be 10pg total rna templates extracted from duck liver samples or 10² copies vira ... | 2013 | 23624117 |