| infection rate of giardia duodenalis, cryptosporidium spp. and enterocytozoon bieneusi in cashmere, dairy and meat goats in china. | cryptosporidiosis, microsporidiosis, and giardiasis contribute significantly to the high burden of zoonotic diarrhea worldwide. goats constitute an important species in animal agriculture by providing cashmere wool, meat, and dairy products for human consumption. however, zoonotic pathogens with the potential to cause morbidity and to degrade production have been reported frequently in goats recently. the present study examined 629 fecal specimens from goats, including 315 cashmere goats, 170 da ... | 2016 | 27017915 |
| first molecular characterization of enteric protozoa and the human pathogenic microsporidian, enterocytozoon bieneusi, in captive snakes in china. | enteric protozoa are frequently found in snakes. nevertheless, few studies regarding genetic characterization of these parasites have been carried out. we describe here the first molecular survey of protozoan pathogens from snakes in china and the first report on enterocytozoon bieneusi genotyping in snakes in the world. here, 240 fecal specimens were collected from two species of captive snakes, naja naja (indian cobra) and ptyas mucosus (oriental rat snake), in guangxi province, china, and exa ... | 2014 | 24906991 |
| occurrence, molecular characterization and predominant genotypes of enterocytozoon bieneusi in dairy cattle in henan and ningxia, china. | enterocytozoon bieneusi is the most frequently diagnosed microsporidian species in humans and a wide range of animals. this study was conducted to assess the prevalence and molecular characteristics of e. bieneusi in dairy cattle in henan province of central china and the ningxia hui autonomous region of northwest china. | 2016 | 26968376 |
| high prevalence and widespread distribution of zoonotic enterocytozoon bieneusi genotypes in swine in northeast china: implications for public health. | this study analyzed 563 fecal specimens of asymptomatic pigs from five cities of northeast china for the prevalence and genetic characteristics of enterocytozoon bieneusi. the parasite was detected in 267 of 563 (47.4%) pigs by nested pcr of the ribosomal internal transcribed spacer (its). the differences in prevalence between preweaned (58.0%, 94/162) and growing pigs (39.6%, 114/288) and between weaned (52.2%, 59/113) and growing pigs are significant (p < 0.05). genotypic typing and phylogenet ... | 2016 | 26333563 |
| zoonotic enterocytozoon bieneusi genotypes in pere david's deer (elaphurus davidianus) in henan, china. | enterocytozoon bieneusi is a zoonotic pathogen of the phylum microspora that infects humans as well as a variety of animal species worldwide. while molecular epidemiologic studies have characterized this parasite in various hosts, isolates from many susceptible hosts have not yet been examined. in this study, e. bieneusi was isolated from 47 pere david's deer (elaphurus davidianus) in henan, china and characterized via pcr analysis of the internal transcribed spacer (its) gene. e. bieneusi was d ... | 2015 | 25982030 |
| predomination and new genotypes of enterocytozoon bieneusi in captive nonhuman primates in zoos in china: high genetic diversity and zoonotic significance. | to appreciate the genetic diversity and zoonotic implications of enterocytozoon bieneusi in nonhuman primates (nhps) in zoos, we genotyped e. bieneusi in captive nhps in seven zoos located at six major cities in china, using ribosomal internal transcribed spacer (its)-based pcr and sequence analyses. a total of 496 fecal specimens from 36 nhp species under nine families were analyzed and e. bieneusi was detected in 148 (29.8%) specimens of 25 nhp species from six families, including cercopitheci ... | 2015 | 25705879 |
| enterocytozoon bieneusi genotypes in children in northeast china and assessment of risk of zoonotic transmission. | the prevalence (7.5%, 19/255) and genotypes of enterocytozoon bieneusi in children of various age categories and clinical presentations were determined herein. the co-occurrence of the known genotypes (cs-4, ebpc, and henan-iv) in children and pigs in the same study area, the phylogenetic characterization of novel genotypes (nec1 to nec5), and the assessment of potential risk factors associated with zoonotic transmission robustly suggested that pigs could be a significant source of human e. bien ... | 2014 | 25274994 |
| genotypic distribution and phylogenetic characterization of enterocytozoon bieneusi in diarrheic chickens and pigs in multiple cities, china: potential zoonotic transmission. | this study investigated diarrheic broiler and layer chickens (<50 days; n=14) and pigs of three age groups (preweaned <30 days, weaned ≈30 to 60 days, and growing >60 days; n=64) for e. bieneusi genotypes in northeast china and evaluated the potential roles of chickens and pigs in zoonotic transmission of microsporidiosis. two 45-day-old layer chickens in city jixi, heilongjiang province and one 23-day-old broiler chicken in city songyuan, jilin province were identified to harbor a human-pathoge ... | 2014 | 25255117 |
| genotypes of enterocytozoon bieneusi in livestock in china: high prevalence and zoonotic potential. | despite many recent advances in genotype characterization of enterocytozoon bieneusi worldwide and the exploration of the extent of cross-species transmission of microsporidiosis between humans and animals, the epidemiology of this neglected disease in china is poorly understood. in this study, a very high prevalence (60.3%; 94/156) of e. bieneusi infections in farmed pigs in jilin province was detected by pcr of the ribosomal internal transcribed spacer (its). dna sequence analysis of 88 e. bie ... | 2014 | 24845247 |
| high diversity of human-pathogenic enterocytozoon bieneusi genotypes in swine in northeast china. | despite the advances in defining enterocytozoon bieneusi genotypes worldwide, rare genotypic surveys have been documented on this ubiquitous pathogenic protozoan in mammals in china, especially the role of pigs in zoonotic transmission of microsporidiosis remains unclear. in this study, the distribution of e. bieneusi genotypes in 113 duodenal mucosal specimens of pigs with acute diarrhea from 15 cities in northeast china was determined by pcr and dna sequence analysis of the ribosomal internal ... | 2014 | 24442159 |
| genetic polymorphism and zoonotic potential of enterocytozoon bieneusi from nonhuman primates in china. | enterocytozoon bieneusi is an important zoonotic pathogen. to assess the human-infective potential of e. bieneusi in nonhuman primates (nhps), we examined the prevalence and genotype distribution of e. bieneusi in 23 nhp species by pcr and sequence analysis of the ribosomal internal transcribed spacer (its). a total of 1,386 fecal specimens from nhps from five provinces in china were examined, and e. bieneusi was detected in 158 (11.4%) specimens from five nhp species, including cynomolgus monke ... | 2014 | 24413605 |