| genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in china. | to characterize the antigenicity of nucleocapsid proteins (np) derived from canine coronavirus (ccov) and canine respiratory coronavirus (crcov) in china, the n genes of ccov (ccov-bj70) and crcov (crcov-bj202) were cloned from swabs obtained from diseased pet dogs in beijing and then sequenced. the recombinant nps (rnps) were expressed in escherichia coli and purified by nickel-affinity column and size exclusion chromatography. sequencing data indicated that the n genes of ccov-bj70 and crcov-b ... | 2016 | 27084706 |