| [isolation, identification and analysis of the complete genome sequence of black queen cell virus strain china-jl1]. | honeybee pupae were collected from jilin apiaries and rna was extracted for use as a tefnplate for amplification. based on the complete genome sequences of black queen cell virus (bqcv) published on genbank, we designed 10 pairs of primers to amplify genes by reverse transcription-polymerase chain reaction (rt-pcr). using this approach, we have obtained the first complete genome sequence of a bqcv isolate in china. the genome of the isolated strain, named bqcv-jl1, is composed of 8358 nucleotide ... | 2015 | 26164935 |